Abstract
Several studies strongly suggest that DC differentiated in vitro in the presence of type I IFN acquire more potent immune stimulatory properties, compared with DC differentiated in vitro with IL-4. However, little is known about the molecular mechanisms underlying this phenomenon. To address this question, we compared the Ag-processing machinery (APM) profile in human DC grown in the presence of IFN-α (IFNDC) or IL-4 (IL-4DC). Using a panel of APM component-specific mAb in Western blot experiments, we found that IFNDC preferentially express inducible proteasome subunits (LMP2, LMP7, and MECL1) both at immature and mature stages. In contrast, immature IL-4DC co-express both constitutive (β1, β2, and β5) and inducible subunits, as shown by Western blotting analysis. In addition, immature IFNDC express higher levels of TAP1, TAP2, calnexin, calreticulin, tapasin, and HLA class I molecules than IL-4DC. The different proteasome profiles of IFNDC and IL-4DC were associated with a greater ability of IFNDC to present an immunodominant epitope that requires LMP7 expression for its processing. In general, these data show the impact of cytokines on APM component expression and hence the Ag-processing ability of DC.
| Original language | English |
|---|---|
| Pages (from-to) | 56-66 |
| Number of pages | 11 |
| Journal | European Journal of Immunology |
| Volume | 39 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Antibodies
- Antigen processing
- Cytokines
- Human DC
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Differential expression of constitutive and inducible proteasome subunits in human monocyte-derived DC differentiated in the presence of IFN-α or IL-4. / Tosello, Valeria; Zamarchi, Rita; Merlo, Anna; Gorza, Margherita; Piovan, Erich; Mandruzzato, Susanna; Bronte, Vincenzo; Wang, Xinhui; Ferrone, Soldano; Amadori, Alberto; Zanovello, Paola.
In: European Journal of Immunology, Vol. 39, No. 1, 2009, p. 56-66.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of constitutive and inducible proteasome subunits in human monocyte-derived DC differentiated in the presence of IFN-α or IL-4
AU - Tosello, Valeria
AU - Zamarchi, Rita
AU - Merlo, Anna
AU - Gorza, Margherita
AU - Piovan, Erich
AU - Mandruzzato, Susanna
AU - Bronte, Vincenzo
AU - Wang, Xinhui
AU - Ferrone, Soldano
AU - Amadori, Alberto
AU - Zanovello, Paola
PY - 2009
Y1 - 2009
N2 - Several studies strongly suggest that DC differentiated in vitro in the presence of type I IFN acquire more potent immune stimulatory properties, compared with DC differentiated in vitro with IL-4. However, little is known about the molecular mechanisms underlying this phenomenon. To address this question, we compared the Ag-processing machinery (APM) profile in human DC grown in the presence of IFN-α (IFNDC) or IL-4 (IL-4DC). Using a panel of APM component-specific mAb in Western blot experiments, we found that IFNDC preferentially express inducible proteasome subunits (LMP2, LMP7, and MECL1) both at immature and mature stages. In contrast, immature IL-4DC co-express both constitutive (β1, β2, and β5) and inducible subunits, as shown by Western blotting analysis. In addition, immature IFNDC express higher levels of TAP1, TAP2, calnexin, calreticulin, tapasin, and HLA class I molecules than IL-4DC. The different proteasome profiles of IFNDC and IL-4DC were associated with a greater ability of IFNDC to present an immunodominant epitope that requires LMP7 expression for its processing. In general, these data show the impact of cytokines on APM component expression and hence the Ag-processing ability of DC.
AB - Several studies strongly suggest that DC differentiated in vitro in the presence of type I IFN acquire more potent immune stimulatory properties, compared with DC differentiated in vitro with IL-4. However, little is known about the molecular mechanisms underlying this phenomenon. To address this question, we compared the Ag-processing machinery (APM) profile in human DC grown in the presence of IFN-α (IFNDC) or IL-4 (IL-4DC). Using a panel of APM component-specific mAb in Western blot experiments, we found that IFNDC preferentially express inducible proteasome subunits (LMP2, LMP7, and MECL1) both at immature and mature stages. In contrast, immature IL-4DC co-express both constitutive (β1, β2, and β5) and inducible subunits, as shown by Western blotting analysis. In addition, immature IFNDC express higher levels of TAP1, TAP2, calnexin, calreticulin, tapasin, and HLA class I molecules than IL-4DC. The different proteasome profiles of IFNDC and IL-4DC were associated with a greater ability of IFNDC to present an immunodominant epitope that requires LMP7 expression for its processing. In general, these data show the impact of cytokines on APM component expression and hence the Ag-processing ability of DC.
KW - Antibodies
KW - Antigen processing
KW - Cytokines
KW - Human DC
UR - http://www.scopus.com/inward/record.url?scp=60549098295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60549098295&partnerID=8YFLogxK
U2 - 10.1002/eji.200738098
DO - 10.1002/eji.200738098
M3 - Article
C2 - 19065646
AN - SCOPUS:60549098295
VL - 39
SP - 56
EP - 66
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 1
ER -