Differential expression of HLA-DR and DR-linked determinants on human leukemias and lymphoid cells

R. A. Newman, D. Delia, M. F. greaves, C. Navarrete, L. Fainboim, H. Festenstein

Research output: Contribution to journalArticle

Abstract

Leukemic and normal hemopoietic cells were examined with the monoclonal antibodies DA2 and Genox 353 for the presence of HLA-DR and DR-linked (DC/MB) determinants, respectively. Although most non-T acute leukemias and leukemic cell lines expressed the monomorphic DR determinant deteced by DA2, fewer than expected expressed the DR-linked polymorphic specificity detected by Genox 353. TdT + lymphoid precursors from normal bone marrow were also DA2 + but Genox 353 -. T cells and thymocytes which were DA2 -, Genox 353 - became DA2 +, Genox 353 + after activation in vitro. Immunoprecipitation using DA2 and Genox 353 gave bands polyacrylamide gel-electrophoresis which were of different molecular weights. In addition, DA2 could absorb out Genox 353 detrminants from a cell lysate whereas Genox 353 could not absorb out DA2 determinants. It is concluded that DA2 and Genox 353 detect HLA-DR and DR-linked (DC1/MB1) determinants, respectively, and that these are differentially expressed on hemopoietic cells during differentiation.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
JournalEuropean Journal of Immunology
Volume13
Issue number2
DOIs
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Immunology

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    Newman, R. A., Delia, D., greaves, M. F., Navarrete, C., Fainboim, L., & Festenstein, H. (1983). Differential expression of HLA-DR and DR-linked determinants on human leukemias and lymphoid cells. European Journal of Immunology, 13(2), 172-176. https://doi.org/10.1002/eji.1830130215