TY - JOUR
T1 - Differential expression of HLA-DR and DR-linked determinants on human leukemias and lymphoid cells
AU - Newman, R. A.
AU - Delia, D.
AU - greaves, M. F.
AU - Navarrete, C.
AU - Fainboim, L.
AU - Festenstein, H.
PY - 1983
Y1 - 1983
N2 - Leukemic and normal hemopoietic cells were examined with the monoclonal antibodies DA2 and Genox 353 for the presence of HLA-DR and DR-linked (DC/MB) determinants, respectively. Although most non-T acute leukemias and leukemic cell lines expressed the monomorphic DR determinant deteced by DA2, fewer than expected expressed the DR-linked polymorphic specificity detected by Genox 353. TdT
+ lymphoid precursors from normal bone marrow were also DA2
+ but Genox 353
-. T cells and thymocytes which were DA2
-, Genox 353
- became DA2
+, Genox 353
+ after activation in vitro. Immunoprecipitation using DA2 and Genox 353 gave bands polyacrylamide gel-electrophoresis which were of different molecular weights. In addition, DA2 could absorb out Genox 353 detrminants from a cell lysate whereas Genox 353 could not absorb out DA2 determinants. It is concluded that DA2 and Genox 353 detect HLA-DR and DR-linked (DC1/MB1) determinants, respectively, and that these are differentially expressed on hemopoietic cells during differentiation.
AB - Leukemic and normal hemopoietic cells were examined with the monoclonal antibodies DA2 and Genox 353 for the presence of HLA-DR and DR-linked (DC/MB) determinants, respectively. Although most non-T acute leukemias and leukemic cell lines expressed the monomorphic DR determinant deteced by DA2, fewer than expected expressed the DR-linked polymorphic specificity detected by Genox 353. TdT
+ lymphoid precursors from normal bone marrow were also DA2
+ but Genox 353
-. T cells and thymocytes which were DA2
-, Genox 353
- became DA2
+, Genox 353
+ after activation in vitro. Immunoprecipitation using DA2 and Genox 353 gave bands polyacrylamide gel-electrophoresis which were of different molecular weights. In addition, DA2 could absorb out Genox 353 detrminants from a cell lysate whereas Genox 353 could not absorb out DA2 determinants. It is concluded that DA2 and Genox 353 detect HLA-DR and DR-linked (DC1/MB1) determinants, respectively, and that these are differentially expressed on hemopoietic cells during differentiation.
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U2 - 10.1002/eji.1830130215
DO - 10.1002/eji.1830130215
M3 - Article
C2 - 6187581
AN - SCOPUS:0020677706
VL - 13
SP - 172
EP - 176
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 2
ER -