Differential expression of multiple transglutaminases in human colon: Impaired keratinocyte transglutaminase expression in ulcerative colitis

G. D'Argenio, M. Calvani, N. Della Valle, V. Cosenza, G. Di Matteo, P. Giorgio, S. Margarucci, O. Petillo, F. P. Jori, U. Galderisi, G. Peluso

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aims: Ulcerative colitis (UC) is characterised by refractory inflammatory ulceration and damage to the colon. The mechanisms underlying impaired healing have yet to be defined. As transglutaminase expression resulting in matrix protein cross linking is associated with increased wound healing in a rat model of colitis, we hypothesised that different types of transglutaminase might also play a role in UC. Patients end methods: Endoscopic and histological indices were studied in 26 patients with UC (10 active and 16 inactive) and in 20 normal controls undergoing colonoscopy. Transglutaminase activity was evaluated in plasma (factor XIIIa) by a radioenzymatic method. Factor XIIIa, tissue and keratinocyte transglutaminase protein content, and mRNA expression in the colon were evaluated by western blot analysis and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively. Colonic location of transglutaminases and their reaction products, the ε-(γ-glutamyl)lysine bonds, was evaluated by immunohistochemistry using specific monoclonal antibodies. Results: Transglutaminase activity was significantly lower in the plasma of patients with active UC (4.2 (2.4) mU/ml; p

Original languageEnglish
Pages (from-to)496-502
Number of pages7
JournalGut
Volume54
Issue number4
DOIs
Publication statusPublished - Apr 2005

ASJC Scopus subject areas

  • Gastroenterology

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