Differential expression of p57(kip2), a maternally imprinted cdk inhibitor, in normal human placenta and gestational trophoblastic disease

Marco Chilosi, Elena Piazzola, Maurizio Lestani, Alice Benedetti, Ilaria Guasparri, Giovanna Granchelli, Daniela Aldovini, Elena Leonardi, Giovanni Pizzolo, Claudio Doglioni, Fabio Menestrina, Gian Mario Mariuzzi

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Evidence has recently been provided to support a role for genomic imprinting in the regulation of embryonic implantation and development and placental growth, as well as in the pathogenesis of proliferative trophoblastic diseases. The cyclin-dependent kinase inhibitor p57(KIP2) has recently been recognized as a maternally imprinted gene. We investigated p57(KIP2) expression in first-trimester normal placentas from interrupted pregnancy, spontaneous abortions, and different types of proliferative trophoblastic diseases using single- and double-marker immunohistochemical techniques. In normal placenta, nuclear p57(KIP2) expression was observed at high frequency (up to 100%) in extravillous trophoblast, cytotrophoblast, and implantation-site interstitial trophoblast, but was absent in syncytiotrophoblast. p57(KIP2) was also expressed in the stromal cells of maternal decidua, which was one of the few adult tissues retaining p57(KIP2) expression (most other adult tissues investigated were negative). p57(KIP2) expression was either absent or low in all cases of diploid/tetraploid complete moles (20 cases) and in three cases of gestational choriocarcinoma. On the other hand, all spontaneous abortions (12 cases) and triploid partial moles (19 cases) showed p57(KIP2) levels comparable to those observed in normal placenta. These findings are in line with the hypothesis that deregulation of genomic imprinting, particularly the loss of cell-cycle inhibitors such as p57(KIP2) is involved in the abnormal development of androgenetic trophoblastic proliferations. In addition, this simple immunohistochemical analysis could provide a useful diagnostic marker in difficult cases.

Original languageEnglish
Pages (from-to)269-276
Number of pages8
JournalLaboratory Investigation
Issue number3
Publication statusPublished - Mar 1998

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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