Differential expression of pentraxin 3 in neutrophils

Olga Razvina, Shuying Jiang, Koichi Matsubara, Riuko Ohashi, Go Hasegawa, Takashi Aoyama, Kenji Daigo, Tatsuhiko Kodama, Takao Hamakubo, Makoto Naito

Research output: Contribution to journalArticlepeer-review

Abstract

Pentraxins belong to the superfamily of conserved proteins that are characterized by a cyclic multimeric structure. Pentraxin 3 (PTX3) is a long pentraxin which can be produced by different cell types upon exposure to various inflammatory signals. Inside the neutrophil PTX3 is stored in form of granules localized in the cytoplasm. Neutrophilic granules are divided into three types: azurophilic (primary) granules, specific (secondary) granules and gelatinase (tertiary) granules. PTX3 has been considered to be localized in specific (secondary) granules. Immunofluorescent analyses using confocal laser microscopic examination were performed to clarify the localization of all three groups of granules within the cytoplasm of the mature neutrophils and neutrophils stimulated with IL-8. Furthermore, PTX3 was localized in primary granules of promyelocyte cell line HL-60. As a result, we suggest that PTX3 is localized not only in specific granules, but is also partly expressed in primary and tertiary granules. After the stimulation with IL-8, irregular reticular structures called neutrophil extracellular traps (NETs) were formed, three types of granules were trapped by NETs and PTX3 showed partial colocalization with these granular components. PTX3 localized in all three types of granules in neutrophils may play important roles in host defense.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalExperimental and Molecular Pathology
Volume98
Issue number1
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • Immunohistochemistry
  • Neutrophil extracellular traps (NETs)
  • Neutrophilic granules
  • Neutrophils
  • Pentraxin 3

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine
  • Medicine(all)

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