Differential expression of specific dermatan sulfate domains in renal pathology

Joost F M Lensen, Johan Van Der Vlag, Elly M M Versteeg, Jack F M Wetzels, Lambert P W J Van Den Heuvel, Jo H M Berden, Toin H. Van Kuppevelt, Angelique L W M M Rops, Maria Pia Rastaldi

Research output: Contribution to journalArticle

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Abstract

Dermatan sulfate (DS), also known as chondroitin sulfate (CS)-B, is a member of the linear polysaccharides called glycosaminoglycans (GAGs). The expression of CS/DS and DS proteoglycans is increased in several fibrotic renal diseases, including interstitial fibrosis, diabetic nephropathy, mesangial sclerosis and nephrosclerosis. Little, however, is known about structural alterations in DS in renal diseases. The aim of this study was to evaluate the renal expression of two different DS domains in renal transplant rejection and glomerular pathologies. DS expression was evaluated in normal renal tissue and in kidney biopsies obtained from patients with acute interstitial or vascular renal allograft rejection, patients with interstitial fibrosis and tubular atrophy (IF/TA), and from patients with focal segmental glomerulosclerosis (FSGS), membranous glomerulopathy (MGP) or systemic lupus erythematosus (SLE), using our unique specific anti-DS antibodies LKN1 and GD3A12. Expression of the 4/2,4-di-O-sulfated DS domain recognized by antibody LKN1 was decreased in the interstitium of transplant kidneys with IF/TA, which was accompanied by an increased expression of type I collagen, decorin and transforming growth factor beta (TGF-β), while its expression was increased in the interstitium in FSGS, MGP and SLE. Importantly, all patients showed glomer-ular LKN1 staining in contrast to the controls. Expression of the IdoA-Gal-NAc4SDS domain recognized by GD3A12 was similar in controls and patients. Our data suggest a role for the DS domain recognized by antibody LKN1 in renal diseases with early fibrosis. Further research is required to delineate the exact role of different DS domains in renal fibrosis.

Original languageEnglish
Article numbere0134946
JournalPLoS One
Volume10
Issue number8
DOIs
Publication statusPublished - Aug 31 2015

Fingerprint

Dermatan Sulfate
Pathology
sulfates
kidneys
Kidney
fibrosis
Fibrosis
kidney diseases
glomerulopathy
Transplants
graft rejection
Focal Segmental Glomerulosclerosis
lupus erythematosus
chondroitin sulfate
kidney transplant
Membranous Glomerulonephritis
atrophy
Antibodies
Systemic Lupus Erythematosus
antibodies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lensen, J. F. M., Van Der Vlag, J., Versteeg, E. M. M., Wetzels, J. F. M., Van Den Heuvel, L. P. W. J., Berden, J. H. M., ... Rastaldi, M. P. (2015). Differential expression of specific dermatan sulfate domains in renal pathology. PLoS One, 10(8), [e0134946]. https://doi.org/10.1371/journal.pone.0134946

Differential expression of specific dermatan sulfate domains in renal pathology. / Lensen, Joost F M; Van Der Vlag, Johan; Versteeg, Elly M M; Wetzels, Jack F M; Van Den Heuvel, Lambert P W J; Berden, Jo H M; Van Kuppevelt, Toin H.; Rops, Angelique L W M M; Rastaldi, Maria Pia.

In: PLoS One, Vol. 10, No. 8, e0134946, 31.08.2015.

Research output: Contribution to journalArticle

Lensen, JFM, Van Der Vlag, J, Versteeg, EMM, Wetzels, JFM, Van Den Heuvel, LPWJ, Berden, JHM, Van Kuppevelt, TH, Rops, ALWMM & Rastaldi, MP 2015, 'Differential expression of specific dermatan sulfate domains in renal pathology', PLoS One, vol. 10, no. 8, e0134946. https://doi.org/10.1371/journal.pone.0134946
Lensen JFM, Van Der Vlag J, Versteeg EMM, Wetzels JFM, Van Den Heuvel LPWJ, Berden JHM et al. Differential expression of specific dermatan sulfate domains in renal pathology. PLoS One. 2015 Aug 31;10(8). e0134946. https://doi.org/10.1371/journal.pone.0134946
Lensen, Joost F M ; Van Der Vlag, Johan ; Versteeg, Elly M M ; Wetzels, Jack F M ; Van Den Heuvel, Lambert P W J ; Berden, Jo H M ; Van Kuppevelt, Toin H. ; Rops, Angelique L W M M ; Rastaldi, Maria Pia. / Differential expression of specific dermatan sulfate domains in renal pathology. In: PLoS One. 2015 ; Vol. 10, No. 8.
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