Differential expression of the GABA(A) receptor complex in the dorsal thalamus and reticular nucleus: An immunohistochemical study in the adult and developing rat

M. Bentivoglio, R. Spreafico, G. Alvarez-Bolado, M. P. Sanchez, A. Fairen

Research output: Contribution to journalArticle

Abstract

The distribution of the GABA(A) receptor/benzodiazepine receptor/chloride channel complex was investigated in the thalamus of the rat by means of immunohistochemistry in adulthood, as well as during embryonic and postnatal development, using a monoclonal antibody. In adults, the immunoreactivity for the GABA(A) receptor complex was intensely expressed by neuronal processes throughout the dorsal thalamus. Neuronal perikaryal membranes were frequently outlined by punctate immunostaining; cell bodies, intrathalamic fibre bundles and the internal capsule did not display immunoreactivity for the GABA(A) receptor. Regional differences in the expression of the receptor were consistently observed: the immunostaining was much lighter in the thalamic reticular nucleus than in the dorsal thalamic nuclei and, among the latter, the anteroventral nucleus and the ventral nuclear complex displayed the most intense immunopositivity. Immunostaining for the GABA(A) receptor was already expressed in embryos at E14, and was homogeneously distributed throughout the neuropil of the dorsal and ventral thalamic primordia. During the first two postnatal weeks, a regional differentiation of the immunopositivity was appreciable in the thalamus, with a progressive reduction in the reticular nucleus and a parallel increase in the dorsal thalamic structures. Immunoreactive neuronal perikarya were not observed in the thalamus at any developmental stage. The expression of the GABA(A) receptor complex appeared to have reached a mature configuration by the end of the third postnatal week. These findings indicate that in adults the GABA(A) receptor is differentially expressed by thalamic nuclear structures, including the reticular nucleus. Furthermore, the maturation of the receptor in the thalamus undergoes a rearrangement during the first postnatal weeks that results in a considerable regression within the reticular nucleus.

Original languageEnglish
Pages (from-to)118-125
Number of pages8
JournalEuropean Journal of Neuroscience
Volume3
Issue number2
Publication statusPublished - 1991

Fingerprint

GABA-A Receptors
Thalamus
Thalamic Nuclei
Anterior Thalamic Nuclei
Internal Capsule
Chloride Channels
Neuropil
Embryonic Development
Embryonic Structures
Immunohistochemistry
Monoclonal Antibodies
Membranes

Keywords

  • Development
  • GABA
  • Immunohistochemistry
  • Inhibition
  • Receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of the GABA(A) receptor complex in the dorsal thalamus and reticular nucleus : An immunohistochemical study in the adult and developing rat. / Bentivoglio, M.; Spreafico, R.; Alvarez-Bolado, G.; Sanchez, M. P.; Fairen, A.

In: European Journal of Neuroscience, Vol. 3, No. 2, 1991, p. 118-125.

Research output: Contribution to journalArticle

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