TY - JOUR
T1 - Differential expression of transforming growth factors-β1, -β2 and -β3 in human colon carcinoma
AU - Bellone, G.
AU - Carbone, A.
AU - Tibaudi, D.
AU - Mauri, F.
AU - Ferrero, I.
AU - Smirne, C.
AU - Suman, F.
AU - Rivetti, C.
AU - Migliaretti, G.
AU - Camandona, M.
AU - Palestro, G.
AU - Emanuelli, G.
AU - Rodeck, U.
PY - 2001
Y1 - 2001
N2 - Transforming growth factor (TGF)-β is a protein family which affects multiple cellular functions including survival, proliferation, differentiation and adhesion. Among the three known isoforms, TGF-β1 is commonly overexpressed in solid malignancies. Recent studies in knock-out mice demonstrated non-redundant roles of different TGF-β isoforms in development. The present study was performed to assess tumour-associated expression of the three TGF-β isoforms in colon carcinoma. We report that colon carcinoma progression is associated with gradual and significant increases in expression of TGF-β1 and TGF-β2 mRNA and proteins. By contrast, TGF-β3 expression was detected in normal colonic mucosa and, at slightly higher levels, in tumour tissues. In addition, plasma levels of both TGF-β1 and TGF-β2 were significantly higher in cancer patients when compared with unaffected individuals. Taken together, our results indicate distinct expression patterns of the three TGF-β isoforms in colon carcinoma cells and possible systemic effects of TGF-β1 and TGF-β2 in tumour patients.
AB - Transforming growth factor (TGF)-β is a protein family which affects multiple cellular functions including survival, proliferation, differentiation and adhesion. Among the three known isoforms, TGF-β1 is commonly overexpressed in solid malignancies. Recent studies in knock-out mice demonstrated non-redundant roles of different TGF-β isoforms in development. The present study was performed to assess tumour-associated expression of the three TGF-β isoforms in colon carcinoma. We report that colon carcinoma progression is associated with gradual and significant increases in expression of TGF-β1 and TGF-β2 mRNA and proteins. By contrast, TGF-β3 expression was detected in normal colonic mucosa and, at slightly higher levels, in tumour tissues. In addition, plasma levels of both TGF-β1 and TGF-β2 were significantly higher in cancer patients when compared with unaffected individuals. Taken together, our results indicate distinct expression patterns of the three TGF-β isoforms in colon carcinoma cells and possible systemic effects of TGF-β1 and TGF-β2 in tumour patients.
KW - Colon carcinoma
KW - Transforming growth factor-β
KW - Tumour progression
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U2 - 10.1016/S0959-8049(00)00391-9
DO - 10.1016/S0959-8049(00)00391-9
M3 - Article
C2 - 11166150
AN - SCOPUS:0035126167
VL - 37
SP - 224
EP - 233
JO - European Journal of Cancer
JF - European Journal of Cancer
SN - 0959-8049
IS - 2
ER -