Differential Expression of Werner and Bloom Syndrome Genes in the Peripheral Blood of HIV-1 Infected Patients

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV)-induced immunodeficiency and immune-system aging share some analogies. Since Werner (WRN) and Bloom (BLM) helicases are crucial in cell repair and aging, their peripheral blood mononuclear cells (PBMC) mRNA levels were compared in HIV-1 infected patients and in normal donors. The mean levels of WRN mRNA were 3.7-fold higher in PBMCs from HIV-1 infected individuals in comparison to healthy donors, whereas BLM mRNA mean levels were slightly higher, although not significantly. WRN increase was positively correlated to CD4 and CD8 T-cell numbers, and also the percentage of naive T lymphocytes, and was observed also in T-cell subsets. Interestingly, a general trend toward increased WRN mRNA levels in individuals with lower viral load was observed, without association with patient age, time of seroconversion, and on/off antiretroviral therapy regimen. On the whole, this study shows that WRN and BLM are differentially modulated in HIV infection, as WRN-but not BLM-is significantly increased, suggesting that mechanisms different from defect or loss of helicase function, observed in WRN and BLM syndromes, may be at the basis of T-cell aging in HIV infection.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalHuman Immunology
Volume68
Issue number2
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Bloom Syndrome
Werner Syndrome
HIV-1
Messenger RNA
Cell Aging
HIV
Virus Diseases
T-Lymphocytes
Genes
Tissue Donors
T-Lymphocyte Subsets
Viral Load
Immune System
Blood Cells
Cell Count

Keywords

  • aging
  • BLM
  • HIV-induced pathogenesis
  • RecQ helicases
  • WRN

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

@article{0f0a2617fef34df1b3c3bc8286d5236e,
title = "Differential Expression of Werner and Bloom Syndrome Genes in the Peripheral Blood of HIV-1 Infected Patients",
abstract = "Human immunodeficiency virus (HIV)-induced immunodeficiency and immune-system aging share some analogies. Since Werner (WRN) and Bloom (BLM) helicases are crucial in cell repair and aging, their peripheral blood mononuclear cells (PBMC) mRNA levels were compared in HIV-1 infected patients and in normal donors. The mean levels of WRN mRNA were 3.7-fold higher in PBMCs from HIV-1 infected individuals in comparison to healthy donors, whereas BLM mRNA mean levels were slightly higher, although not significantly. WRN increase was positively correlated to CD4 and CD8 T-cell numbers, and also the percentage of naive T lymphocytes, and was observed also in T-cell subsets. Interestingly, a general trend toward increased WRN mRNA levels in individuals with lower viral load was observed, without association with patient age, time of seroconversion, and on/off antiretroviral therapy regimen. On the whole, this study shows that WRN and BLM are differentially modulated in HIV infection, as WRN-but not BLM-is significantly increased, suggesting that mechanisms different from defect or loss of helicase function, observed in WRN and BLM syndromes, may be at the basis of T-cell aging in HIV infection.",
keywords = "aging, BLM, HIV-induced pathogenesis, RecQ helicases, WRN",
author = "Licia Bordi and Cristiana Gioia and Eleonora Lalle and Pierluca Piselli and Fabrizio Poccia and Capobianchi, {Maria R.} and Alessandra Amendola",
year = "2007",
month = "2",
doi = "10.1016/j.humimm.2006.11.004",
language = "English",
volume = "68",
pages = "91--99",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Differential Expression of Werner and Bloom Syndrome Genes in the Peripheral Blood of HIV-1 Infected Patients

AU - Bordi, Licia

AU - Gioia, Cristiana

AU - Lalle, Eleonora

AU - Piselli, Pierluca

AU - Poccia, Fabrizio

AU - Capobianchi, Maria R.

AU - Amendola, Alessandra

PY - 2007/2

Y1 - 2007/2

N2 - Human immunodeficiency virus (HIV)-induced immunodeficiency and immune-system aging share some analogies. Since Werner (WRN) and Bloom (BLM) helicases are crucial in cell repair and aging, their peripheral blood mononuclear cells (PBMC) mRNA levels were compared in HIV-1 infected patients and in normal donors. The mean levels of WRN mRNA were 3.7-fold higher in PBMCs from HIV-1 infected individuals in comparison to healthy donors, whereas BLM mRNA mean levels were slightly higher, although not significantly. WRN increase was positively correlated to CD4 and CD8 T-cell numbers, and also the percentage of naive T lymphocytes, and was observed also in T-cell subsets. Interestingly, a general trend toward increased WRN mRNA levels in individuals with lower viral load was observed, without association with patient age, time of seroconversion, and on/off antiretroviral therapy regimen. On the whole, this study shows that WRN and BLM are differentially modulated in HIV infection, as WRN-but not BLM-is significantly increased, suggesting that mechanisms different from defect or loss of helicase function, observed in WRN and BLM syndromes, may be at the basis of T-cell aging in HIV infection.

AB - Human immunodeficiency virus (HIV)-induced immunodeficiency and immune-system aging share some analogies. Since Werner (WRN) and Bloom (BLM) helicases are crucial in cell repair and aging, their peripheral blood mononuclear cells (PBMC) mRNA levels were compared in HIV-1 infected patients and in normal donors. The mean levels of WRN mRNA were 3.7-fold higher in PBMCs from HIV-1 infected individuals in comparison to healthy donors, whereas BLM mRNA mean levels were slightly higher, although not significantly. WRN increase was positively correlated to CD4 and CD8 T-cell numbers, and also the percentage of naive T lymphocytes, and was observed also in T-cell subsets. Interestingly, a general trend toward increased WRN mRNA levels in individuals with lower viral load was observed, without association with patient age, time of seroconversion, and on/off antiretroviral therapy regimen. On the whole, this study shows that WRN and BLM are differentially modulated in HIV infection, as WRN-but not BLM-is significantly increased, suggesting that mechanisms different from defect or loss of helicase function, observed in WRN and BLM syndromes, may be at the basis of T-cell aging in HIV infection.

KW - aging

KW - BLM

KW - HIV-induced pathogenesis

KW - RecQ helicases

KW - WRN

UR - http://www.scopus.com/inward/record.url?scp=33847150200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847150200&partnerID=8YFLogxK

U2 - 10.1016/j.humimm.2006.11.004

DO - 10.1016/j.humimm.2006.11.004

M3 - Article

C2 - 17321898

AN - SCOPUS:33847150200

VL - 68

SP - 91

EP - 99

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 2

ER -