Differential gene expression of dopamine D-2 receptor subtypes in rat chromaffin cells and sympathetic neurons in culture

Sandra Sigala, Cristina Missale, Nadia Tognazzi, PierFranco Spano

Research output: Contribution to journalArticlepeer-review

Abstract

Chromaffin cells and sympathetic neurons arise from a common bipotential progenitor which, if exposed to nerve growth factor (NGF), matures into a sympathetic neuron, but if exposed to glucocorticoids (GCs), differentiates into a mature chromaffin cell. Pharmacological evidence indicates that, in adrenal medulla and sympathetic neurons, dopamine (DA) receptors belonging to the D-2 family inhibit catecholamine secretion. The molecular characterization of these receptors, however, is not been yet described. Our data suggest that bipotential cells obtained from newborn rat adrenal medulla express both isoforms of the D-2 receptor, while D-3 receptor and D-4 receptor messenger RNAs (mRNAs) are not present. GC-mediated maturation induces the expression of D-4 receptors, without modification of D-2 isoforms. Sympathetic neurons differentiated in vitro selectively express the D-2short mRNA. Taken together, present results suggest that NGF and GCs play a role in regulating D-2 family receptor expression in neural crest-derived cells. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)2467-2471
Number of pages5
JournalNeuroReport
Volume11
Issue number11
Publication statusPublished - Aug 3 2000

Keywords

  • Dopamine
  • Glucocorticoids
  • Nerve growth factor
  • Neural-crest derived cells
  • Receptors

ASJC Scopus subject areas

  • Neuroscience(all)

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