TY - JOUR
T1 - Differential growth of N- and S-type human neuroblastoma cells xenografted into SCID mice. Correlation with apoptosis
AU - Piacentini, Mauro
AU - Piredda, Lucia
AU - Starace, Donatella
AU - Annicchiarico-Petruzzelli, Margherita
AU - Mattei, Maurizio
AU - Oliverio, Serafina
AU - Farrace, Maria Grazia
AU - Melino, Gerry
PY - 1996
Y1 - 1996
N2 - This study concerns tire role of apoptosis in the growth of human neuroblastomas transplanted into immunodeficient SCID mice. Human neuroblastoma cell lines may consist of one or more distinct phenotypes including the neural 'N-type' and flat substrate-adherent 'S-type'. A differential phenotype-specific proliferation was apparent among S- and N-type cell clones transplanted into SCTD mice when compared with the mild-type SK-N-BE(2) cell line. This differential growth capacity of the tumours was correlated with spontaneous apoptosis. Another SK-N-BE(2)-derived cell line (TGA), displaying high levels of apoptosis upon stable transfection with the full length 'tissue' transglutaminase (tTG) cDNA, was unable to indnce tumour development when xenografted into SCID mice. To support these observations, the expression of apoptosis-related genes (i.e., bcl-2, p53, and tTG) in the various neuroblastomas was also investigated.
AB - This study concerns tire role of apoptosis in the growth of human neuroblastomas transplanted into immunodeficient SCID mice. Human neuroblastoma cell lines may consist of one or more distinct phenotypes including the neural 'N-type' and flat substrate-adherent 'S-type'. A differential phenotype-specific proliferation was apparent among S- and N-type cell clones transplanted into SCTD mice when compared with the mild-type SK-N-BE(2) cell line. This differential growth capacity of the tumours was correlated with spontaneous apoptosis. Another SK-N-BE(2)-derived cell line (TGA), displaying high levels of apoptosis upon stable transfection with the full length 'tissue' transglutaminase (tTG) cDNA, was unable to indnce tumour development when xenografted into SCID mice. To support these observations, the expression of apoptosis-related genes (i.e., bcl-2, p53, and tTG) in the various neuroblastomas was also investigated.
KW - Apoptosis
KW - bcl-2
KW - Tissue transglutaminase
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U2 - 10.1002/(SICI)1096-9896(199612)180:4<415::AID-PATH684>3.0.CO;2-A
DO - 10.1002/(SICI)1096-9896(199612)180:4<415::AID-PATH684>3.0.CO;2-A
M3 - Article
C2 - 9014863
AN - SCOPUS:0030470053
VL - 180
SP - 415
EP - 422
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 4
ER -