Differential in vitro modulation of suppressor and antitumor functions of mouse macrophages by lymphokines and/or endotoxin

Zong Liang Chang, Ezio Bonvini, Luigi Varesio, Howard T. Holden, Ronald B. Herberman

Research output: Contribution to journalArticle

Abstract

Peritoneal macrophages of normal mice exhibited natural suppressor activity, as indicated by their ability to inhibit the proliferation of spleen cells in response to stimulation with phytohemagglutinin (PHA) or concanavalin A (Con A). Their suppressor function could be modulated in vitro with a variety of treatment regimens. High-dose lipopolysaccharide (LPS) (LPSH; 10 μg/ ml) or lymphokines (supernatant from Con A-stimulated spleen cells) plus low-dose LPS (LPSL; 10 ng/ml) caused a reduction in the suppressor activity of adherent peritoneal macrophages. In contrast, these same treatments induced the macrophages to become tumoricidal and cytostatic for tumor cells, indicating a major dissociation between the regulation of suppressor and cytotoxic activities of macrophages. The lack of correlation between these activities was further demonstrated by macrophages that had been activated in vitro by Corynebacterium parvum: these cells expressed high tumoricidal and cytostatic activities, and also strong suppressor activity. The suppressor function could be selectively downregulated by in vitro pretreatment with LPSH.

Original languageEnglish
Pages (from-to)282-292
Number of pages11
JournalCellular Immunology
Volume114
Issue number2
DOIs
Publication statusPublished - 1988

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Lymphokines
Endotoxins
Macrophages
Peritoneal Macrophages
Cytostatic Agents
Concanavalin A
Lipopolysaccharides
Spleen
Propionibacterium acnes
Phytohemagglutinins
Down-Regulation
Cell Proliferation
In Vitro Techniques
Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Differential in vitro modulation of suppressor and antitumor functions of mouse macrophages by lymphokines and/or endotoxin. / Chang, Zong Liang; Bonvini, Ezio; Varesio, Luigi; Holden, Howard T.; Herberman, Ronald B.

In: Cellular Immunology, Vol. 114, No. 2, 1988, p. 282-292.

Research output: Contribution to journalArticle

Chang, Zong Liang ; Bonvini, Ezio ; Varesio, Luigi ; Holden, Howard T. ; Herberman, Ronald B. / Differential in vitro modulation of suppressor and antitumor functions of mouse macrophages by lymphokines and/or endotoxin. In: Cellular Immunology. 1988 ; Vol. 114, No. 2. pp. 282-292.
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