Differential induction of the two early genes c-jun and c-fos in weakly and strongly metastatic murine lymphoma cell lines

D. Rossi, S. Fumagalli, L. Volpi, L. Larizza

Research output: Contribution to journalArticle

Abstract

Induction of the 2 early-response genes c-jun and c-fos was investigated in the weakly metastatic T-lymphoma Eb line and the related strongly metastatic lymphomacrophage ESb line to find possible correlations with their different in vitro and in vivo phenotypes. The response of c-jun was elicited by the protein kinase-C activators TPA and A23187 in ESb but not in Eb cells. A much lower response of c-fos was also found in Eb than in ESb cells, in this case by means of serum and the cAMP elevator forskolin. However, both TPA and the calcium ionophore A23187 were similarly effective in inducing fos-mRNA in both cell lines. The uncoupling of c-jun and c-fos induction in Eb, but not in ESb cells, as well as the uncoupling of c-fos response to different stimulators, point to a differential activation of these 2 early-response genes by the main signal transduction pathways in the 2 cell types. The coordinate/uncoordinate availability of the fos/jun heterodimer may confer distinct regulatory patterns on different target genes in ESb/Eb cells. Activation of these genes may underlie the distinct differentiation phenotypes and in vivo behavior of Eb/ESb cells.

Original languageEnglish
Pages (from-to)86-89
Number of pages4
JournalInternational Journal of Cancer
Volume57
Issue number1
Publication statusPublished - 1994

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jun Genes
Lymphoma
Cell Line
Calcimycin
Elevators and Escalators
Phenotype
Calcium Ionophores
Colforsin
Protein Kinase C
Transcriptional Activation
Genes
Signal Transduction
Messenger RNA
Serum

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Differential induction of the two early genes c-jun and c-fos in weakly and strongly metastatic murine lymphoma cell lines. / Rossi, D.; Fumagalli, S.; Volpi, L.; Larizza, L.

In: International Journal of Cancer, Vol. 57, No. 1, 1994, p. 86-89.

Research output: Contribution to journalArticle

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