TY - JOUR
T1 - Differential mRNA expression in untreated and TNF-α elicited murine dendritic cells precursors
AU - Rovere, Patrizia
AU - Trucy, Jeannine
AU - Zimmermann, Valérie S.
AU - Granjeaud, Samuel
AU - Rocha, Dominique
AU - Nguyen, Catherine
AU - Ricciardi-Castagnoli, Paola
AU - Jordan, Bertrand R.
AU - Davoust, Jean
PY - 1997
Y1 - 1997
N2 - We have compared the pattern of gene expression in long term cultured precursor dendritic cells (DC), either untreated (immature) or cultured for two days in the presence of recombinant murine (rm)-TNFα1 (mature). The hybridization signature of complex cDNA probes prepared from total RNA extracted from immature and mature DC were analyzed using a mouse thymic cDNA library, gridded on high density filters. For each clone spotted on the filters, we have measured using an imaging plate device the hybridization signals of the complex probe obtained from immature or mature DC. Comparative analysis of these values allowed us to identify differentially expressed gene products. Our goal is to identify a new set of genes induced or repressed during DC maturation elicited by rmTNFα treatment.
AB - We have compared the pattern of gene expression in long term cultured precursor dendritic cells (DC), either untreated (immature) or cultured for two days in the presence of recombinant murine (rm)-TNFα1 (mature). The hybridization signature of complex cDNA probes prepared from total RNA extracted from immature and mature DC were analyzed using a mouse thymic cDNA library, gridded on high density filters. For each clone spotted on the filters, we have measured using an imaging plate device the hybridization signals of the complex probe obtained from immature or mature DC. Comparative analysis of these values allowed us to identify differentially expressed gene products. Our goal is to identify a new set of genes induced or repressed during DC maturation elicited by rmTNFα treatment.
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M3 - Article
C2 - 9286405
AN - SCOPUS:0030846607
VL - 417
SP - 467
EP - 473
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -