Differential patterns of expression of Eps15 and Eps15R during mouse embryogenesis

Nina Offenhäuser, Elisa Santolini, Antonio Simeone, Pier Paolo Di Fiore

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Eps15 and Eps15R are related tyrosine kinase substrates, which have been implicated in endocytosis and synaptic vesicle recycling. Through the protein:protein interaction abilities of their EH domains, they establish a complex network of interactions with several proteins, including Numb, a protein necessary for neuronal cell fate specification. We analyzed the expression of Eps15 and Eps15R during murine development, at the time of active neurogenesis. The most striking difference was at the level of subcellular localization, with Eps15 present in the cytosol and on the plasma membrane, while Eps15R exhibited mainly a nuclear localization. Interesting topographical differences also emerged. In the 12.5 days post coitum neuroepithelium, Eps15 was expressed in the ventricular zone, which contains proliferating neuroblasts, whereas Eps15R was found only in postmitotic neurons. Conversely, both proteins were expressed in sensory and cranial ganglia. At later times, the expression of Eps15 and Eps15R was widely maintained in neuronal structures. In other tissues, Eps15 was first seen in the liver primordium and at low levels in choroid plexus, lung, kidney and intestine; later on the expression was maintained at high levels in epithelia. Nuclear staining of Eps15R was present in kidney, intestine, lung and liver, as well as in heart and pancreas. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)309-312
Number of pages4
JournalMechanisms of Development
Volume95
Issue number1-2
DOIs
Publication statusPublished - Jul 1 2000

Fingerprint

Embryonic Development
Proteins
Intestines
Sensory Ganglia
Kidney
Lung
Choroid Plexus
Synaptic Vesicles
Liver
Neurogenesis
Endocytosis
Cytosol
Protein-Tyrosine Kinases
Pancreas
Epithelium
Cell Membrane
Staining and Labeling
Neurons

Keywords

  • Eps15
  • Eps15R
  • Expression
  • Immunohistochemistry
  • Mouse embryo
  • Neurogenesis
  • Numb

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Differential patterns of expression of Eps15 and Eps15R during mouse embryogenesis. / Offenhäuser, Nina; Santolini, Elisa; Simeone, Antonio; Di Fiore, Pier Paolo.

In: Mechanisms of Development, Vol. 95, No. 1-2, 01.07.2000, p. 309-312.

Research output: Contribution to journalArticle

Offenhäuser, Nina ; Santolini, Elisa ; Simeone, Antonio ; Di Fiore, Pier Paolo. / Differential patterns of expression of Eps15 and Eps15R during mouse embryogenesis. In: Mechanisms of Development. 2000 ; Vol. 95, No. 1-2. pp. 309-312.
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