Abstract
The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein repertoire for the development of improved methods to differentiate X and Y sperm cells maintaining high vitality. In order to address this issue, we performed a comparative shotgun proteomic investigation by nUPLC-MS/MS to characterize sexed bovine semen. The protein profiles of these two types of sperm cells have shown differential expression of proteins that may be directly associated with the main components of cytoskeletal structures of flagellum, as the axoneme, outer dense fibers and fibrous sheath, as well as glycolytic enzymes and calmodulin, involved in the energetic metabolism regulation. Overall these results may provide a base to a better comprehension of the biological features of sperm cells and may be useful to the development of alternative methods of separation.
| Original language | English |
|---|---|
| Pages (from-to) | 1264-1271 |
| Number of pages | 8 |
| Journal | Molecular BioSystems |
| Volume | 10 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 |
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ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Medicine(all)
Cite this
Differential protein profile in sexed bovine semen : Shotgun proteomics investigation. / De Canio, Michele; Soggiu, Alessio; Piras, Cristian; Bonizzi, Luigi; Galli, Andrea; Urbani, Andrea; Roncada, Paola.
In: Molecular BioSystems, Vol. 10, No. 6, 2014, p. 1264-1271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential protein profile in sexed bovine semen
T2 - Shotgun proteomics investigation
AU - De Canio, Michele
AU - Soggiu, Alessio
AU - Piras, Cristian
AU - Bonizzi, Luigi
AU - Galli, Andrea
AU - Urbani, Andrea
AU - Roncada, Paola
PY - 2014
Y1 - 2014
N2 - The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein repertoire for the development of improved methods to differentiate X and Y sperm cells maintaining high vitality. In order to address this issue, we performed a comparative shotgun proteomic investigation by nUPLC-MS/MS to characterize sexed bovine semen. The protein profiles of these two types of sperm cells have shown differential expression of proteins that may be directly associated with the main components of cytoskeletal structures of flagellum, as the axoneme, outer dense fibers and fibrous sheath, as well as glycolytic enzymes and calmodulin, involved in the energetic metabolism regulation. Overall these results may provide a base to a better comprehension of the biological features of sperm cells and may be useful to the development of alternative methods of separation.
AB - The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein repertoire for the development of improved methods to differentiate X and Y sperm cells maintaining high vitality. In order to address this issue, we performed a comparative shotgun proteomic investigation by nUPLC-MS/MS to characterize sexed bovine semen. The protein profiles of these two types of sperm cells have shown differential expression of proteins that may be directly associated with the main components of cytoskeletal structures of flagellum, as the axoneme, outer dense fibers and fibrous sheath, as well as glycolytic enzymes and calmodulin, involved in the energetic metabolism regulation. Overall these results may provide a base to a better comprehension of the biological features of sperm cells and may be useful to the development of alternative methods of separation.
UR - http://www.scopus.com/inward/record.url?scp=84898767864&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898767864&partnerID=8YFLogxK
U2 - 10.1039/c3mb70306a
DO - 10.1039/c3mb70306a
M3 - Article
C2 - 24226273
AN - SCOPUS:84898767864
VL - 10
SP - 1264
EP - 1271
JO - Molecular BioSystems
JF - Molecular BioSystems
SN - 1742-206X
IS - 6
ER -