Differential proteome analysis of replicative senescence in rat embryo fibroblasts.

Silvia Benvenuti, Rainer Cramer, Christopher C. Quinn, Jim Bruce, Marketa Zvelebil, Steven Corless, Jacquelyn Bond, Alice Yang, Susan Hockfield, Alma L. Burlingame, Michael D. Waterfield, Parmjit S. Jat

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Normal somatic cells undergo a finite number of divisions and then cease dividing whereas cancer cells are able to proliferate indefinitely. To identify the underlying mechanisms that limit the mitotic potential, a two-dimensional differential proteome analysis of replicative senescence in serially passaged rat embryo fibroblasts was undertaken. Triplicate independent two-dimensional gels containing over 1200 spots each were run, curated, and analyzed. This revealed 49 spots whose expression was altered more than 2-fold. Of these, 42 spots yielded positive protein identification by mass spectrometry comprising a variety of cytoskeletal, heat shock, and metabolic proteins, as well as proteins involved in trafficking, differentiation, and protein synthesis, turnover, and modification. These included gelsolin, a candidate tumor suppressor for breast cancer, and alpha-glucosidase II, a member of the family of glucosidases that includes klotho; a defect in klotho expression in mice results in a syndrome that resembles human aging. Changes in expression of TUC-1, -2, -4, and -4 beta, members of the TUC family critical for neuronal differentiation, were also identified. Some of the identified changes were also shown to occur in two other models of senescence, premature senescence of REF52 cells and replicative senescence of mouse embryo fibroblasts. The majority of these candidate proteins were unrecognized previously in replicative senescence. They are now implicated in a new role.

Original languageEnglish
Pages (from-to)280-292
Number of pages13
JournalMolecular and Cellular Proteomics
Volume1
Issue number4
Publication statusPublished - Apr 2002

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Cell Aging
Proteome
Fibroblasts
Rats
Embryonic Structures
Proteins
Gelsolin
Glucosidases
alpha-Glucosidases
Protein Transport
Heat-Shock Proteins
Mass Spectrometry
Neoplasms
Gels
Mass spectrometry
Breast Neoplasms
Tumors
Aging of materials
Cells
Defects

ASJC Scopus subject areas

  • Biochemistry

Cite this

Benvenuti, S., Cramer, R., Quinn, C. C., Bruce, J., Zvelebil, M., Corless, S., ... Jat, P. S. (2002). Differential proteome analysis of replicative senescence in rat embryo fibroblasts. Molecular and Cellular Proteomics, 1(4), 280-292.

Differential proteome analysis of replicative senescence in rat embryo fibroblasts. / Benvenuti, Silvia; Cramer, Rainer; Quinn, Christopher C.; Bruce, Jim; Zvelebil, Marketa; Corless, Steven; Bond, Jacquelyn; Yang, Alice; Hockfield, Susan; Burlingame, Alma L.; Waterfield, Michael D.; Jat, Parmjit S.

In: Molecular and Cellular Proteomics, Vol. 1, No. 4, 04.2002, p. 280-292.

Research output: Contribution to journalArticle

Benvenuti, S, Cramer, R, Quinn, CC, Bruce, J, Zvelebil, M, Corless, S, Bond, J, Yang, A, Hockfield, S, Burlingame, AL, Waterfield, MD & Jat, PS 2002, 'Differential proteome analysis of replicative senescence in rat embryo fibroblasts.', Molecular and Cellular Proteomics, vol. 1, no. 4, pp. 280-292.
Benvenuti S, Cramer R, Quinn CC, Bruce J, Zvelebil M, Corless S et al. Differential proteome analysis of replicative senescence in rat embryo fibroblasts. Molecular and Cellular Proteomics. 2002 Apr;1(4):280-292.
Benvenuti, Silvia ; Cramer, Rainer ; Quinn, Christopher C. ; Bruce, Jim ; Zvelebil, Marketa ; Corless, Steven ; Bond, Jacquelyn ; Yang, Alice ; Hockfield, Susan ; Burlingame, Alma L. ; Waterfield, Michael D. ; Jat, Parmjit S. / Differential proteome analysis of replicative senescence in rat embryo fibroblasts. In: Molecular and Cellular Proteomics. 2002 ; Vol. 1, No. 4. pp. 280-292.
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