Abstract
The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTMs, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNAs is altered.
| Original language | English |
|---|---|
| Pages (from-to) | 1220-1227 |
| Number of pages | 8 |
| Journal | Proteomics |
| Volume | 13 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Apr 2013 |
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Keywords
- Biomedicine
- Diamond-Blackfan anemia
- DIGE
- Erythropoiesis
- Ribosomal proteins
- RPS19
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry
Cite this
Differential proteomic analysis in human cells subjected to ribosomal stress. / Caterino, Marianna; Corbo, Claudia; Imperlini, Esther; Armiraglio, Marta; Pavesi, Elisa; Aspesi, Anna; Loreni, Fabrizio; Dianzani, Irma; Ruoppolo, Margherita.
In: Proteomics, Vol. 13, No. 7, 04.2013, p. 1220-1227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential proteomic analysis in human cells subjected to ribosomal stress
AU - Caterino, Marianna
AU - Corbo, Claudia
AU - Imperlini, Esther
AU - Armiraglio, Marta
AU - Pavesi, Elisa
AU - Aspesi, Anna
AU - Loreni, Fabrizio
AU - Dianzani, Irma
AU - Ruoppolo, Margherita
PY - 2013/4
Y1 - 2013/4
N2 - The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTMs, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNAs is altered.
AB - The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTMs, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNAs is altered.
KW - Biomedicine
KW - Diamond-Blackfan anemia
KW - DIGE
KW - Erythropoiesis
KW - Ribosomal proteins
KW - RPS19
UR - http://www.scopus.com/inward/record.url?scp=84875905978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875905978&partnerID=8YFLogxK
U2 - 10.1002/pmic.201200242
DO - 10.1002/pmic.201200242
M3 - Article
C2 - 23412928
AN - SCOPUS:84875905978
VL - 13
SP - 1220
EP - 1227
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 7
ER -