Differential proteomic analysis in human cells subjected to ribosomal stress

Marianna Caterino, Claudia Corbo, Esther Imperlini, Marta Armiraglio, Elisa Pavesi, Anna Aspesi, Fabrizio Loreni, Irma Dianzani, Margherita Ruoppolo

Research output: Contribution to journalArticlepeer-review

Abstract

The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTMs, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNAs is altered.

Original languageEnglish
Pages (from-to)1220-1227
Number of pages8
JournalProteomics
Volume13
Issue number7
DOIs
Publication statusPublished - Apr 2013

Keywords

  • Biomedicine
  • Diamond-Blackfan anemia
  • DIGE
  • Erythropoiesis
  • Ribosomal proteins
  • RPS19

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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