Differential regulation of chemokine production by Fcγ receptor engagement in human monocytes: Association of CCL1 with a distinct form of M2 monocyte activation (M2b, Type 2)

Marina Sironi, Fernando O. Martinez, Daniele D'Ambrosio, Marco Gattorno, Nadia Polentarutti, Massimo Locati, Andrea Gregorio, Andrea Iellem, Marco A. Cassatella, Jo Van Damme, Silvano Sozzani, Alberto Martini, Francesco Sinigaglia, Annunciata Vecchi, Alberto Mantovani

Research output: Contribution to journalArticle

Abstract

CC chemokine ligand 1 (CCL1; I-309) is a CC chemokine that interacts with CC chemokine receptor 8, which is preferentially expressed in polarized T helper cell type 2 and Tc2 cells, in eosinophils, and in T regulatory cells. The present study, prompted by transcriptional profiling of human monocytes undergoing different forms of activation, was designed to characterize the production of CCL1 in monocytes compared with the production of other chemokines (CCL2, CCL22, and CCL18) differentially regulated by distinct activation signals. Lipopolysaccharide (LPS), interferon-γ (IFN-γ), interleukin (IL)-1β, tumor necrosis factor α, IL-4, IL-13, IL-10, IL-6, IL-18, and combinations thereof did not induce CCL1 production in monocytes, and some of these signals stimulated production of reference chemokines. Induction of CCL1 in monocytes required engagement of Fc receptor for immunoglobulin G (FcγR)II and exposure to IL-1β or LPS. This combination of stimuli results in a form of M2 (M2b, Type 2) macrophage activation. FcγR engagement also induced CCL22 and amplified its stimulation by IL-4. In contrast, FcγR stimulation inhibited the IL-10- and LPS-mediated induction of CCL18. IL-10, IL-4, and IFN-γ inhibited induction of CCL1 by FcγR ligation and IL-1β. CCL1 was present in synovial fluids and macrophages in juvenile idiopathic arthritis. Thus, regulation of CCL1 in human monocytes is unique, with an obligate requirement of FcγR engagement and costimulation by signals (IL-1β and LPS), which use the myeloid differentiation primary-response protein 88 adaptor protein. Thus, CCL1 is a CC chemokine with a unique pattern of regulation associated with a distinct form of M2 (Type 2, M2b) monocyte activation, which participates in macrophage-dependent regulatory circuits of innate and adaptive immunity.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalJournal of Leukocyte Biology
Volume80
Issue number2
DOIs
Publication statusPublished - Aug 2006

Keywords

  • CCR8
  • IL-1
  • MyD88
  • Th2

ASJC Scopus subject areas

  • Cell Biology

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