Differential regulation of Epstein-Barr virus (EBV) latent gene expression in burkitt lymphoma cells infected with a recombinant ebv strain

P. Trivedi, P. Spinsanti, L. Cuomo, M. Volpe, K. Takada, L. Frati, A. Faggioni

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus (EBV)-negative Burkitt lymphomas (BLs) can be infected in vitro with prototype EBV strains to study how the virus may affect the phenotype of tumor cells. Studies thus far have concentrated on the use of transforming B95-8 and nontransforming P3HR1 strains. Immunological and phenotypic differences between the sublines infected with these two strains were reported. The majority of these differences, if not all, can be attributed to the lack of EBNA-2 coding sequences in the P3HR1 strain. The recent development of a selectable Akata strain has opened up new possibilities for infecting epithelial and T cells as well. We infected five EBV-negative BL lines with the recombinant Akata virus. Our results indicate that the infected cell lines BL28, Ramos, and DG75 express EBNA-1, EBNA-2, and LMP1, the viral proteins associated with type III latency, and use both YUK and QUK splices. In contrast, two EBV-negative variants of Akata and Mutu when reinfected displayed restricted type I latency and expressed only EBNA-1. All clones of infected Mutu cells used the QUK splice exclusively. The usage of Qp was observed in a majority of Akata clones. Some Akata clones, however, were found to have double promoter usage (Qp and C/Wp) but at 4 months after infection did not express EBNA-2. The results demonstrate differential regulation of EBV latency in BLs with the same recombinant viral strain and suggest that the choice of latency type may be cell dependent. The restricted latency observed for infected Akata and Mutu cells indicates that a BL may opt for type I latency in the absence of immune pressure as well.

Original languageEnglish
Pages (from-to)4929-4935
Number of pages7
JournalJournal of Virology
Volume75
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Human herpesvirus 4
Burkitt Lymphoma
lymphoma
Human Herpesvirus 4
Gene Expression
gene expression
Clone Cells
cells
clones
Virus Latency
Viruses
Viral Proteins
viruses
viral proteins
prototypes
Epithelial Cells
epithelial cells
T-Lymphocytes
Phenotype
Pressure

ASJC Scopus subject areas

  • Immunology

Cite this

Differential regulation of Epstein-Barr virus (EBV) latent gene expression in burkitt lymphoma cells infected with a recombinant ebv strain. / Trivedi, P.; Spinsanti, P.; Cuomo, L.; Volpe, M.; Takada, K.; Frati, L.; Faggioni, A.

In: Journal of Virology, Vol. 75, No. 10, 2001, p. 4929-4935.

Research output: Contribution to journalArticle

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