Abstract
Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
| Original language | English |
|---|---|
| Pages (from-to) | 11-17 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 221 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 31 1987 |
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Keywords
- (Human hepatoma cell line)
- Epidermal growth factor receptor
- Hepatitis B virus
- Insulin receptor
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens. / Clementi, Massimo; Testa, Ivano; Festa, Antonella; Bagnarelli, Patrizia; Chang, Chungming; Carloni, Guido.
In: FEBS Letters, Vol. 221, No. 1, 31.08.1987, p. 11-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens
AU - Clementi, Massimo
AU - Testa, Ivano
AU - Festa, Antonella
AU - Bagnarelli, Patrizia
AU - Chang, Chungming
AU - Carloni, Guido
PY - 1987/8/31
Y1 - 1987/8/31
N2 - Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
AB - Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
KW - (Human hepatoma cell line)
KW - Epidermal growth factor receptor
KW - Hepatitis B virus
KW - Insulin receptor
UR - http://www.scopus.com/inward/record.url?scp=0023223709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023223709&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(87)80343-5
DO - 10.1016/0014-5793(87)80343-5
M3 - Article
VL - 221
SP - 11
EP - 17
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -