Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens

Massimo Clementi; Ivano Testa; Antonella Festa; Patrizia Bagnarelli; Chungming Chang; Guido Carloni

doi:10.1016/0014-5793(87)80343-5

Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens

Massimo Clementi, Ivano Testa, Antonella Festa, Patrizia Bagnarelli, Chungming Chang, Guido Carloni

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.

Original language	English
Pages (from-to)	11-17
Number of pages	7
Journal	FEBS Letters
Volume	221
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80343-5
Publication status	Published - Aug 31 1987

Keywords

(Human hepatoma cell line)
Epidermal growth factor receptor
Hepatitis B virus
Insulin receptor

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(87)80343-5

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abstract = "Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.",

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AU - Clementi, Massimo

AU - Testa, Ivano

AU - Festa, Antonella

AU - Bagnarelli, Patrizia

AU - Chang, Chungming

AU - Carloni, Guido

PY - 1987/8/31

Y1 - 1987/8/31

N2 - Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.

AB - Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.

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KW - Epidermal growth factor receptor

KW - Hepatitis B virus

KW - Insulin receptor

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Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens

Abstract

Keywords

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