Differential responsiveness of proliferation and cytokeratin release to stripped serum and oestrogen in the human breast cancer cell line, MCF-7

T. Tedone, M. Correale, A. Paradiso, S. J. Reshkin

Research output: Contribution to journalArticle

Abstract

In vitro research into hormone sensitivity and the relation to proliferation of cytokeratin release from cancer cells is scarce. Therefore, we examined the stimulation of proliferation and the release of cytokeratins in a breast cancer cell culture model. Cell growth was stimulated by 17β-oestradiol (10-11 M), stripped serum (10%) and by the two together. Cytokeratin release was stimulated only by stripped serum, oestradiol having no effect. After long incubation periods (>12 h), cytokeratin release also commenced in the control and oestradiol treatments. Release rate versus time analysis suggested that there are two different release processes. Cytokeratin release was first stimulated at a stripped serum concentration approximately 100 times lower than that which initiated proliferation. Pharmacological alteration of proliferation with cordyceptin resulted in growth changes without alterations in cytokeratin release. We conclude that cytokeratin release in these cells is unrelated to proliferation, independent of oestrogen action and probably in some way related to growth factor receptor function.

Original languageEnglish
Pages (from-to)849-856
Number of pages8
JournalEuropean Journal of Cancer
Volume32
Issue number5
DOIs
Publication statusPublished - May 1996

Keywords

  • Breast cancer
  • CYFRA 21.1
  • Cytokeratins
  • Growth factors
  • Hormone
  • MCF-7
  • Oestrogen
  • Proliferation
  • TPA
  • TPS

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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