Differential responsiveness to constitutive vs. inducible chemokines of immature and mature mouse dendritic cells

Annunciata Vecchi, Lucia Massimiliano, Simona Ramponi, Walter Luini, Sergio Bernasconi, Raffaella Bonecchi, Paola Allavena, Marc Parmentier, Alberto Mantovani, Silvano Sozzani

Research output: Contribution to journalArticlepeer-review

Abstract

Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultured with granulocyte-macrophage-CSF and Flt3 ligand for 9 days. Chemokines active on immature DC include MIP1α, RANTES, MIP1β, MCP-1, MCP-3, and the constitutively expressed SDF1, MDC, and ELC. TNF-α-induced DC maturation caused reduction of migration to inducible chemokines (MIP1α, RANTES, MIP1β, MCP-1, and MCP-3) and increased migration to SDF1, MDC, and ELC. Similar results were obtained by CD40 ligation or culture in the presence of bacterial lipopolysaccharide. TNF-α down- regulated CC chemokine receptor (CCR)1, CCR2, and CCR5 and up-regulated CCR7 mRNA levels, in agreement with functional data. This study shows that selective responsiveness of mature and immature DC to inducible vs. constitutively produced chemokines can contribute to the regulated trafficking of DC.

Original languageEnglish
Pages (from-to)489-494
Number of pages6
JournalJournal of Leukocyte Biology
Volume66
Issue number3
Publication statusPublished - 1999

Keywords

  • CD34-derived DC
  • Chemokime receptors
  • Chemotaxis
  • Transmigration

ASJC Scopus subject areas

  • Cell Biology

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