Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultured with granulocyte-macrophage-CSF and Flt3 ligand for 9 days. Chemokines active on immature DC include MIP1α, RANTES, MIP1β, MCP-1, MCP-3, and the constitutively expressed SDF1, MDC, and ELC. TNF-α-induced DC maturation caused reduction of migration to inducible chemokines (MIP1α, RANTES, MIP1β, MCP-1, and MCP-3) and increased migration to SDF1, MDC, and ELC. Similar results were obtained by CD40 ligation or culture in the presence of bacterial lipopolysaccharide. TNF-α down- regulated CC chemokine receptor (CCR)1, CCR2, and CCR5 and up-regulated CCR7 mRNA levels, in agreement with functional data. This study shows that selective responsiveness of mature and immature DC to inducible vs. constitutively produced chemokines can contribute to the regulated trafficking of DC.
|Number of pages||6|
|Journal||Journal of Leukocyte Biology|
|Publication status||Published - 1999|
- CD34-derived DC
- Chemokime receptors
ASJC Scopus subject areas
- Cell Biology