Abstract
PCAF is a histone acetyltransferase that associates with p300/CBP and competes with E1A for access to them. While exogenous expression of PCAF potentiates both MyoD-directed transcription and myogenic differentiation, PCAF inactivation by anti-PCAF antibody microinjection prevents differentiation. MyoD interacts directly with both p300/CBP and PCAF, forming a multimeric protein complex on the promoter elements. Viral transforming factors that interfere with muscle differentiation disrupt this complex without affecting the MyoD-DNA interaction, indicating functional significance of the complex formation. Exogenous expression of PCAF or p300 promotes p21 expression and terminal cell-cycle arrest. Both of these activities are dependent on the histone acetyltransferase activity of PCAF, but not on that of p300. These results indicate that recruitment of histone acetyltransferase activity of PCAF by MyoD, through p300/CBP, is crucial for activation of the myogenic program.
| Original language | English |
|---|---|
| Pages (from-to) | 35-45 |
| Number of pages | 11 |
| Journal | Molecular Cell |
| Volume | 1 |
| Issue number | 1 |
| Publication status | Published - Dec 1997 |
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- Molecular Biology
Cite this
Differential roles of p300 and PCAF acetyltransferases in muscle differentiation. / Puri, Pier Lorenzo; Sartorelli, Vittorio; Yang, Xiang Jiao; Hamamori, Yasuo; Ogryzko, Vasily V.; Howard, Bruce H.; Kedes, Larry; Wang, Jean Y J; Graessmann, Adolf; Nakatani, Yoshihiro; Levrero, Massimo.
In: Molecular Cell, Vol. 1, No. 1, 12.1997, p. 35-45.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential roles of p300 and PCAF acetyltransferases in muscle differentiation
AU - Puri, Pier Lorenzo
AU - Sartorelli, Vittorio
AU - Yang, Xiang Jiao
AU - Hamamori, Yasuo
AU - Ogryzko, Vasily V.
AU - Howard, Bruce H.
AU - Kedes, Larry
AU - Wang, Jean Y J
AU - Graessmann, Adolf
AU - Nakatani, Yoshihiro
AU - Levrero, Massimo
PY - 1997/12
Y1 - 1997/12
N2 - PCAF is a histone acetyltransferase that associates with p300/CBP and competes with E1A for access to them. While exogenous expression of PCAF potentiates both MyoD-directed transcription and myogenic differentiation, PCAF inactivation by anti-PCAF antibody microinjection prevents differentiation. MyoD interacts directly with both p300/CBP and PCAF, forming a multimeric protein complex on the promoter elements. Viral transforming factors that interfere with muscle differentiation disrupt this complex without affecting the MyoD-DNA interaction, indicating functional significance of the complex formation. Exogenous expression of PCAF or p300 promotes p21 expression and terminal cell-cycle arrest. Both of these activities are dependent on the histone acetyltransferase activity of PCAF, but not on that of p300. These results indicate that recruitment of histone acetyltransferase activity of PCAF by MyoD, through p300/CBP, is crucial for activation of the myogenic program.
AB - PCAF is a histone acetyltransferase that associates with p300/CBP and competes with E1A for access to them. While exogenous expression of PCAF potentiates both MyoD-directed transcription and myogenic differentiation, PCAF inactivation by anti-PCAF antibody microinjection prevents differentiation. MyoD interacts directly with both p300/CBP and PCAF, forming a multimeric protein complex on the promoter elements. Viral transforming factors that interfere with muscle differentiation disrupt this complex without affecting the MyoD-DNA interaction, indicating functional significance of the complex formation. Exogenous expression of PCAF or p300 promotes p21 expression and terminal cell-cycle arrest. Both of these activities are dependent on the histone acetyltransferase activity of PCAF, but not on that of p300. These results indicate that recruitment of histone acetyltransferase activity of PCAF by MyoD, through p300/CBP, is crucial for activation of the myogenic program.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031310741&partnerID=8YFLogxK
M3 - Article
C2 - 9659901
AN - SCOPUS:0031310741
VL - 1
SP - 35
EP - 45
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 1
ER -