Differential roles of p300 and PCAF acetyltransferases in muscle differentiation

Pier Lorenzo Puri, Vittorio Sartorelli, Xiang Jiao Yang, Yasuo Hamamori, Vasily V. Ogryzko, Bruce H. Howard, Larry Kedes, Jean Y J Wang, Adolf Graessmann, Yoshihiro Nakatani, Massimo Levrero

Research output: Contribution to journalArticle

Abstract

PCAF is a histone acetyltransferase that associates with p300/CBP and competes with E1A for access to them. While exogenous expression of PCAF potentiates both MyoD-directed transcription and myogenic differentiation, PCAF inactivation by anti-PCAF antibody microinjection prevents differentiation. MyoD interacts directly with both p300/CBP and PCAF, forming a multimeric protein complex on the promoter elements. Viral transforming factors that interfere with muscle differentiation disrupt this complex without affecting the MyoD-DNA interaction, indicating functional significance of the complex formation. Exogenous expression of PCAF or p300 promotes p21 expression and terminal cell-cycle arrest. Both of these activities are dependent on the histone acetyltransferase activity of PCAF, but not on that of p300. These results indicate that recruitment of histone acetyltransferase activity of PCAF by MyoD, through p300/CBP, is crucial for activation of the myogenic program.

Original languageEnglish
Pages (from-to)35-45
Number of pages11
JournalMolecular Cell
Volume1
Issue number1
Publication statusPublished - Dec 1997

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Differential roles of p300 and PCAF acetyltransferases in muscle differentiation'. Together they form a unique fingerprint.

  • Cite this

    Puri, P. L., Sartorelli, V., Yang, X. J., Hamamori, Y., Ogryzko, V. V., Howard, B. H., Kedes, L., Wang, J. Y. J., Graessmann, A., Nakatani, Y., & Levrero, M. (1997). Differential roles of p300 and PCAF acetyltransferases in muscle differentiation. Molecular Cell, 1(1), 35-45.