Differential sensitivity to (dl)-5-methyltetrahydrofolate of normal CFU-GM and HL-60 cells

Valter Gattei; Pietro Antonio Bernabei; Pierluigi Rossi Ferrini

doi:10.1016/0145-2126(89)90126-4

Differential sensitivity to (dl)-5-methyltetrahydrofolate of normal CFU-GM and HL-60 cells

Valter Gattei, Pietro Antonio Bernabei, Pierluigi Rossi Ferrini

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10^-4 to 5 × 10^-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10^-4 and 1 × 10^-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.

Original language	English
Pages (from-to)	595-598
Number of pages	4
Journal	Leukemia Research
Volume	13
Issue number	7
DOIs	https://doi.org/10.1016/0145-2126(89)90126-4
Publication status	Published - 1989

Keywords

5-methyltetrahydrofolate
cell proliferation
CFU-GM
HL-60 cells

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

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abstract = "We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10-4 to 5 × 10-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10-4 and 1 × 10-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating {"}folate{"} transport in normal and leukemic cells.",

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AU - Bernabei, Pietro Antonio

AU - Ferrini, Pierluigi Rossi

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Y1 - 1989

N2 - We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10-4 to 5 × 10-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10-4 and 1 × 10-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.

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