Differential sensitivity to (dl)-5-methyltetrahydrofolate of normal CFU-GM and HL-60 cells

Valter Gattei, Pietro Antonio Bernabei, Pierluigi Rossi Ferrini

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Abstract

We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10-4 to 5 × 10-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10-4 and 1 × 10-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.

Original languageEnglish
Pages (from-to)595-598
Number of pages4
JournalLeukemia Research
Volume13
Issue number7
DOIs
Publication statusPublished - 1989

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Keywords

  • 5-methyltetrahydrofolate
  • cell proliferation
  • CFU-GM
  • HL-60 cells

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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