TY - JOUR
T1 - Differential sensitivity to (dl)-5-methyltetrahydrofolate of normal CFU-GM and HL-60 cells
AU - Gattei, Valter
AU - Bernabei, Pietro Antonio
AU - Ferrini, Pierluigi Rossi
PY - 1989
Y1 - 1989
N2 - We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10-4 to 5 × 10-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10-4 and 1 × 10-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.
AB - We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 × 10-4 to 5 × 10-4 M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 × 10-4 and 1 × 10-3 M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.
KW - 5-methyltetrahydrofolate
KW - cell proliferation
KW - CFU-GM
KW - HL-60 cells
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U2 - 10.1016/0145-2126(89)90126-4
DO - 10.1016/0145-2126(89)90126-4
M3 - Article
C2 - 2761291
AN - SCOPUS:0024383062
VL - 13
SP - 595
EP - 598
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 7
ER -