Differential susceptibility to modulation by recombinant immune interferon of HLA-DR and -DQ antigens synthesized by melanoma COLO 38 cells

P. Giacomini, A. Aguzzi, S. Ferrone

Research output: Contribution to journalArticle

Abstract

Recombinant immune interferon (IFN-γ) induced a dose-related increase in the synthesis and expression of HLA class II antigens by the cultured melanoma cells COLO 38. Although IFN-γ-treated melanoma cells COLO 38 continue to express higher levels of HLA-DR antigens than of HLA-DQ antigens, the effect of IFN-γ was more marked on HLA-DQ antigens than on HLA-DR antigens, as indicated by the dose and incubation time required to induce the changes and by the extent and duration of the increase. The effect of IFN-γ on HLA class II antigens is significantly higher than that of leukocyte and fibroblast interferons. Analysis by one- and two-dimensional gel electrophoresis of antigens synthesized by melanoma cells COLO 38 in the presence of IFN-γ did not detect any significant change in the structural profile of the subunits of HLA-DR and -DQ antigens.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalHybridoma
Volume5
Issue number4
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Genetics
  • Immunology

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