Differential therapeutic effect of extracellular vesicles derived by bone marrow and adipose mesenchymal stem cells on wound healing of diabetic ulcers and correlation to their cargoes

Margherita Pomatto, Chiara Gai, Federica Negro, Massimo Cedrino, Cristina Grange, Elena Ceccotti, Gabriele Togliatto, Federica Collino, Marta Tapparo, Federico Figliolini, Tatiana Lopatina, Maria Felice Brizzi, Giovanni Camussi

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular vesicles (EVs) derived from mesenchymal stem cells isolated from both bone marrow (BMSCs) and adipose tissue (ADSCs) show potential therapeutic effects. These vesicles often show a similar beneficial effect on tissue regeneration, but in some contexts, they exert different biological properties. To date, a comparison of their molecular cargo that could explain the different biological effect is not available. Here, we demonstrated that ADSC-EVs, and not BMSC-EVs, promote wound healing on a murine model of diabetic wounds. Besides a general similarity, the bioinformatic analysis of their protein and miRNA cargo highlighted important differences between these two types of EVs. Molecules present exclusively in ADSC-EVs were highly correlated to angiogenesis, whereas those expressed in BMSC-EVs were preferentially involved in cellular proliferation. Finally, in vitro analysis confirmed that both ADSC and BMSC-EVs exploited beneficial effect on cells involved in skin wound healing such as fibroblasts, keratinocytes and endothelial cells, but through different cellular processes. Consistent with the bioinformatic analyses, BMSC-EVs were shown to mainly promote proliferation, whereas ADSC-EVs demonstrated a major effect on angiogenesis. Taken together, these results provide deeper comparative information on the cargo of ADSC-EVs and BMSC-EVs and the impact on regenerative processes essential for diabetic wound healing.

Original languageEnglish
Article number3851
JournalInternational Journal of Molecular Sciences
Volume22
Issue number8
DOIs
Publication statusPublished - Apr 2 2021

Keywords

  • Adipose
  • Bone marrow
  • Cargo
  • Diabetes
  • Exosome
  • Extracellular vesicle
  • Mesenchymal
  • MSC
  • Therapy
  • Wound healing

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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