Differentiation and migration properties of human foetal umbilical cord perivascular cells: Potential for lung repair

Tiziana Montemurro, Gabriella Andriolo, Elisa Montelatici, Gaia Weissmann, Mihaela Crisan, Maria Rosa Colnaghi, Paolo Rebulla, Fabio Mosca, Bruno Péault, Lorenza Lazzari

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSC) have been derived from different cultured human tissues, including bone marrow, adipose tissue, amniotic fluid and umbilical cord blood. Only recently it was suggested that MSC descended from perivascular cells, the latter being defined as CD146+ neuro-glial proteoglycan (NG)2+ platelet-derived growth factor-Rβ+ ALP+ CD34- CD45- von Willebrand factor (vWF)- CD144-. Herein we studied the properties of perivascular cells from a novel source, the foetal human umbilical cord (HUC) collected from pre-term newborns. By immunohistochemistry and flow cytometry we show that pre-term/foetal HUCs contain more perivascular cells than their full-term counterparts (2.5%versus 0.15%). Moreover, foetal HUC perivascular cells (HUCPC) express the embryonic cell markers specific embryonic antigen-4, Runx1 and Oct-4 and can be cultured over the long term. To further confirm the MSC identity of these cultured perivascular cells, we also showed their expression at different passages of antigens that typify MSC. The multilineage differentiative capacity of HUCPC into osteogenic, adipogenic and myogenic cell lineages was demonstrated in culture. In the perspective of a therapeutic application in chronic lung disease of pre-term newborns, we demonstrated the in vitro ability of HUCPC to migrate towards an alveolar type II cell line damaged with bleomycin, an anti-cancer agent with known pulmonary toxicity. The secretory profile exhibited by foetal HUCPC in the migration assay suggested a paracrine effect that could be exploited in various clinical conditions including lung disorders.

Original languageEnglish
Pages (from-to)796-808
Number of pages13
JournalJournal of Cellular and Molecular Medicine
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 2011

Keywords

  • Lung repair
  • Newborns
  • Pericytes
  • Umbilical cords

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

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