Differentiation between non-Hodgkin's lymphoma recurrence and second primary lymphoma by VDJ rearrangement analysis

Massimo Libra, Valli De Re, Daniela Gasparotto, Annunziata Gloghini, Alessandra Marzotto, Isabella Milan, Umberto Tirelli, Franca Stivala, Antonino Carbone, Mauro Boiocchi

Research output: Contribution to journalArticle


Relapses in non-Hodgkin's lymphomas (NHL) could be due to the reappearance of the initial neoplasm or new primary tumours. Discrimination between the two events would allow a more targeted therapeutic approach. VDJ rearrangement was used as marker of clonality in metachronous biopsy specimens from 10 patients with relapsed B-NHL. Complimentarity determining region 3 was amplified and sequenced. D-JH was identical in eight matched primary/secondary tumours, confirming the diagnosis of recurrence. In contrast, primary and secondary tumours in two patients were of different clonal origin. Our data indicate that VDJ analysis is a fundamental tool for identificaton of relapses in NHL.

Original languageEnglish
Pages (from-to)809-812
Number of pages4
JournalBritish Journal of Haematology
Issue number3
Publication statusPublished - 2002



  • non-Hodgkin's lymphoma
  • Relapse
  • VDJ

ASJC Scopus subject areas

  • Hematology

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