Differentiation dependent expression in muscle cells of ZT3, a novel zinc finger factor differentially expressed in embryonic and adult tissues

M. Polimeni, S. Giorgi, L. De Gregorio, T. A. Dragani, M. Molinaro, G. Cossu, M. Bouché

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

ZT3, isolated from a murine muscle cell cDNA library by a low-stringency hybridization, encodes a zinc finger domain containing factor with a transcript of 5.0 kb. A 3' 2.5 kb partial nucleotide sequence contains an ORF of 1.5 kb where 17 canonical C2H2 zinc finger domains organized in tandem were identified. It maps on mouse chromosome 11, close to two mutations which affect skeletal formation. ZT3 expression depends upon differentiation of myogenic cells in culture, since it is upregulated with myogenin and inhibited in scr-transfected C2C12 cells. ZT3 is not expressed in NIH3T3 or C3H10T1/2 fibroblasts, but is induced when fibroblasts are myogenically converted by transfection with the muscle regulatory genes (MRFs). Its expression is also upregulated in the rhabdomyosarcoma cell line RD induced to myogenic differentiation by TPA treatment. In postimplantation embryos, ZT3 is diffusely expressed but higher expression is detectable in the neural tube and encephalic vesicles, in the somites and, at a high level, in the limb buds as they form. During further development ZT3 is expressed in many tissues of neuroectodermal and mesodermal origin, but its expression decreases during fetal development and in the adult it is restricted to skeletal and cardiac muscle and to spleen. This pattern of expression suggests a possible role played by ZT3 in differentiating skeletal muscle. Its expression in other tissues is compatible with the suggestion that members of this class of DNA-binding factors play different roles during post-implantation development and in the adult life.

Original languageEnglish
Pages (from-to)107-117
Number of pages11
JournalMechanisms of Development
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 1996

Fingerprint

Zinc Fingers
Muscle Cells
Skeletal Muscle
Fibroblasts
Myogenin
Limb Buds
Somites
Chromosomes, Human, Pair 11
Neural Tube
Rhabdomyosarcoma
Regulator Genes
Fetal Development
Gene Library
Open Reading Frames
Transfection
Myocardium
Spleen
Embryonic Structures
Cell Culture Techniques
Cell Line

Keywords

  • Chromosome mapping
  • Gene expression
  • Muscle development
  • Muscle differentiation
  • Regulatory factors
  • Zinc finger proteins

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Differentiation dependent expression in muscle cells of ZT3, a novel zinc finger factor differentially expressed in embryonic and adult tissues. / Polimeni, M.; Giorgi, S.; De Gregorio, L.; Dragani, T. A.; Molinaro, M.; Cossu, G.; Bouché, M.

In: Mechanisms of Development, Vol. 54, No. 1, 01.1996, p. 107-117.

Research output: Contribution to journalArticle

Polimeni, M. ; Giorgi, S. ; De Gregorio, L. ; Dragani, T. A. ; Molinaro, M. ; Cossu, G. ; Bouché, M. / Differentiation dependent expression in muscle cells of ZT3, a novel zinc finger factor differentially expressed in embryonic and adult tissues. In: Mechanisms of Development. 1996 ; Vol. 54, No. 1. pp. 107-117.
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