Differentiation-linked expression of cell surface markers on HL-60 leukemic cells

M. A. Boss, D. Delia, J. B. Robinson, M. F. Greaves

Research output: Contribution to journalArticle

Abstract

The cell surface antigenic phenotype of HL-60, a human acute promyelocytic leukemia cell line, has been analyzed before and after maturation induction with dimethylsulfoxide (DMSO) using a panel of markers including a 'library' of monoclonal antibodies and 'conventional' antisera in conjunction with the fluorescence-activated cell sorter. HL-60 cells express granulocytes and 'leukocyte' differentiation antigens but not antigens of the lymphoid, platelet, and erythroid lineages. DMSO-induced morphological maturation was found to be associated with a decrease in the proportion of cells in mitotic cycle, induction of C3d receptors, increased expression of granulocytic and leukocyte antigens, and diminished expression of HLA-A,B,C and β2-microglobulin determinants. HL-60 cells have no detectable expression of HLA-DR-associated determinants as assayed by rabbit anti-p28,33 monoclonal anti-HLA-DR (monomorphic determinant), and HLA-DRw typing alloantisera. The relationship of these changes in cell surface properties to normal granulocytic differentiation is discussed.

Original languageEnglish
Pages (from-to)910-916
Number of pages7
JournalBlood
Volume56
Issue number5
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Hematology

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    Boss, M. A., Delia, D., Robinson, J. B., & Greaves, M. F. (1980). Differentiation-linked expression of cell surface markers on HL-60 leukemic cells. Blood, 56(5), 910-916.