Differentiation of diverse progenies of memory T cells from naïve CD8+ T cell precursors

Veronica Zanon, Enrico Lugli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Following recognition of the cognate antigen, naïve T cells differentiate in a diverse progeny of memory T cells which differ at the phenotypic, gene expression and metabolic level. These molecular differences are at the basis of discrete functionality, migratory capacity and persistence in the long-term. Such a division of labor benefits adoptive T cell transfer immunotherapy approaches for cancer and viral infections, as increased persistence and effector functions in vivo result in better control of the disease. Preclinical data suggest that early-differentiated T memory stem cells are the most powerful anti-tumor T cell population following adoptive transfer, but their paucity ex vivo limits translation to the clinic. Here, we describe a simple protocol to derive large numbers of T memory stem cell and effector CD8+ T cell subsets from highly-purified CD8+ naïve T cell precursors. The obtained cells can be studied in vitro to understand the molecular basis of human memory T cell differentiation, or, when redirected with T cell receptor or chimeric antigen receptor, potentially used in vivo to favour T cell reconstitution or to treat established tumors or chronic infections.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages103-110
Number of pages8
Volume1514
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1514
ISSN (Print)10643745

Keywords

  • Adoptive cell transfer
  • Cancer
  • CD8 T cells
  • Cytokines
  • Effector T cells
  • Immunotherapy
  • Memory
  • Naïve
  • T memory stem cells
  • TCR stimulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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