Differentiation of human bone marrow stromal cells onto gelatin cryogel scaffolds

L. Fassina, E. Saino, L. Visai, M. A. Avanzini, M. G Cusella De Angelis, F. Benazzo, S. Van Vlierberghe, P. Dubruel, G. Magenes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiating medium, the use of a differentiating medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiating medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages9-12
Number of pages4
Volume29
DOIs
Publication statusPublished - 2010
Event12th Mediterranean Conference on Medical and Biological Engineering and Computing, MEDICON 2010 - Chalkidiki, Greece
Duration: May 27 2010May 30 2010

Other

Other12th Mediterranean Conference on Medical and Biological Engineering and Computing, MEDICON 2010
CountryGreece
CityChalkidiki
Period5/27/105/30/10

Keywords

  • biomimetics
  • bone extracellular matrix
  • bone marrow stromal cell
  • cell proliferation
  • Gelatin cryogel
  • surface modification

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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  • Cite this

    Fassina, L., Saino, E., Visai, L., Avanzini, M. A., De Angelis, M. G. C., Benazzo, F., Van Vlierberghe, S., Dubruel, P., & Magenes, G. (2010). Differentiation of human bone marrow stromal cells onto gelatin cryogel scaffolds. In IFMBE Proceedings (Vol. 29, pp. 9-12) https://doi.org/10.1007/978-3-642-13039-7_3