Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose Ara-C

Monitoring by expression of c-myc and c-fos oncogenes

Antonio Pinto, Vincenza Attadia, Rita Rosati, Giulia Colletta, Renato Cimino, Alfonso Colombatti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of low dose Ara-C on cellular oncogene expression were measured in HL-60 and U-937 cell lines and in primary cultures from leukaemic patients. Expression of c-myc was completely abolished in U-937 and greatly reduced in HL-60 after a 3 day exposure to the drug, whereas specific c-fos transcripts were increased. In fresh myeloid leukaemia samples, growth and DNA synthesis were reduced as in the two cell lines. Conversely, changes compatible with the induction of differentiation along the myelomonocytic pathway were much less pronounced than in cell lines treated with the same dose of Ara-C. Cells from one patient did not show any appreciable morphological change. The same sample displayed a greatly reduced expression of c-myc accompanied by a concurrent 10-fold increased expression of c-fos. The data suggest that the action of low dose Ara-C on oncogene expression is comparable to that of other differentiation-inducing agents that display both cytostatic and maturation promoting effects. Evaluation of cellular oncogene expression may therefore represent a useful tool for monitoring effects of low dose Ara-C on leukaemia cells.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalMedical Oncology and Tumor Pharmacotherapy
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1988

Fingerprint

Cytarabine
Oncogenes
Leukemia
Cell Line
Myeloid Leukemia
Cytostatic Agents
DNA
Growth
Pharmaceutical Preparations

Keywords

  • Acute leukaemia
  • Differentiation
  • Low dose Ara-C
  • Oncogenes

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)
  • Oncology

Cite this

Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose Ara-C : Monitoring by expression of c-myc and c-fos oncogenes. / Pinto, Antonio; Attadia, Vincenza; Rosati, Rita; Colletta, Giulia; Cimino, Renato; Colombatti, Alfonso.

In: Medical Oncology and Tumor Pharmacotherapy, Vol. 5, No. 2, 06.1988, p. 91-97.

Research output: Contribution to journalArticle

Pinto, Antonio ; Attadia, Vincenza ; Rosati, Rita ; Colletta, Giulia ; Cimino, Renato ; Colombatti, Alfonso. / Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose Ara-C : Monitoring by expression of c-myc and c-fos oncogenes. In: Medical Oncology and Tumor Pharmacotherapy. 1988 ; Vol. 5, No. 2. pp. 91-97.
@article{ea267511e5ad4037994272ad00e33e0c,
title = "Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose Ara-C: Monitoring by expression of c-myc and c-fos oncogenes",
abstract = "The effects of low dose Ara-C on cellular oncogene expression were measured in HL-60 and U-937 cell lines and in primary cultures from leukaemic patients. Expression of c-myc was completely abolished in U-937 and greatly reduced in HL-60 after a 3 day exposure to the drug, whereas specific c-fos transcripts were increased. In fresh myeloid leukaemia samples, growth and DNA synthesis were reduced as in the two cell lines. Conversely, changes compatible with the induction of differentiation along the myelomonocytic pathway were much less pronounced than in cell lines treated with the same dose of Ara-C. Cells from one patient did not show any appreciable morphological change. The same sample displayed a greatly reduced expression of c-myc accompanied by a concurrent 10-fold increased expression of c-fos. The data suggest that the action of low dose Ara-C on oncogene expression is comparable to that of other differentiation-inducing agents that display both cytostatic and maturation promoting effects. Evaluation of cellular oncogene expression may therefore represent a useful tool for monitoring effects of low dose Ara-C on leukaemia cells.",
keywords = "Acute leukaemia, Differentiation, Low dose Ara-C, Oncogenes",
author = "Antonio Pinto and Vincenza Attadia and Rita Rosati and Giulia Colletta and Renato Cimino and Alfonso Colombatti",
year = "1988",
month = "6",
doi = "10.1007/BF02985444",
language = "English",
volume = "5",
pages = "91--97",
journal = "Medical Oncology",
issn = "1357-0560",
publisher = "Humana Press Inc.",
number = "2",

}

TY - JOUR

T1 - Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose Ara-C

T2 - Monitoring by expression of c-myc and c-fos oncogenes

AU - Pinto, Antonio

AU - Attadia, Vincenza

AU - Rosati, Rita

AU - Colletta, Giulia

AU - Cimino, Renato

AU - Colombatti, Alfonso

PY - 1988/6

Y1 - 1988/6

N2 - The effects of low dose Ara-C on cellular oncogene expression were measured in HL-60 and U-937 cell lines and in primary cultures from leukaemic patients. Expression of c-myc was completely abolished in U-937 and greatly reduced in HL-60 after a 3 day exposure to the drug, whereas specific c-fos transcripts were increased. In fresh myeloid leukaemia samples, growth and DNA synthesis were reduced as in the two cell lines. Conversely, changes compatible with the induction of differentiation along the myelomonocytic pathway were much less pronounced than in cell lines treated with the same dose of Ara-C. Cells from one patient did not show any appreciable morphological change. The same sample displayed a greatly reduced expression of c-myc accompanied by a concurrent 10-fold increased expression of c-fos. The data suggest that the action of low dose Ara-C on oncogene expression is comparable to that of other differentiation-inducing agents that display both cytostatic and maturation promoting effects. Evaluation of cellular oncogene expression may therefore represent a useful tool for monitoring effects of low dose Ara-C on leukaemia cells.

AB - The effects of low dose Ara-C on cellular oncogene expression were measured in HL-60 and U-937 cell lines and in primary cultures from leukaemic patients. Expression of c-myc was completely abolished in U-937 and greatly reduced in HL-60 after a 3 day exposure to the drug, whereas specific c-fos transcripts were increased. In fresh myeloid leukaemia samples, growth and DNA synthesis were reduced as in the two cell lines. Conversely, changes compatible with the induction of differentiation along the myelomonocytic pathway were much less pronounced than in cell lines treated with the same dose of Ara-C. Cells from one patient did not show any appreciable morphological change. The same sample displayed a greatly reduced expression of c-myc accompanied by a concurrent 10-fold increased expression of c-fos. The data suggest that the action of low dose Ara-C on oncogene expression is comparable to that of other differentiation-inducing agents that display both cytostatic and maturation promoting effects. Evaluation of cellular oncogene expression may therefore represent a useful tool for monitoring effects of low dose Ara-C on leukaemia cells.

KW - Acute leukaemia

KW - Differentiation

KW - Low dose Ara-C

KW - Oncogenes

UR - http://www.scopus.com/inward/record.url?scp=0023890828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023890828&partnerID=8YFLogxK

U2 - 10.1007/BF02985444

DO - 10.1007/BF02985444

M3 - Article

VL - 5

SP - 91

EP - 97

JO - Medical Oncology

JF - Medical Oncology

SN - 1357-0560

IS - 2

ER -