Dihydrotestosterone and bicalutamide do not affect periostin expression in androgen-dependent LNCaP prostate cancer cell lines

Francesca Argellati, Pier Vitale Nuzzo, Francesco Ricci, Rosa Mangerini, Alessandra Rubagotti, Francesco Boccardo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background/Aim: To investigate periostin (POSTN) expression in the LNCaP cell line. Materials and Methods: Our LNCaP strain did not constitutively express the POSTN gene. Through cell transfection with a cloning vector, we developed an LNCaP cell line that stably expressed POSTN. LNCaP wild-type and transfected cells were incubated with dihydrotestosterone (DHT) in the presence/or absence of bicalutamide (BIC). POSTN mRNA was detected by quantitative real-time reverse transcriptionpolymerase chain reaction (qRT-PCR) and growth was measured with the MTT assay. Results: POSTN transfection stimulated LNCaP cell growth. While POSTN transfection did not interfere with the stimulatory effect of DHT, BIC had an inhibitory effect on cell proliferation. However, exposure to either DHT and/or BIC was not able to interfere with POSTN expression per se. Conclusion: We confirmed the role of POSTN in promoting cancer cell growth. Although POSTN transcription is not likely to be androgen-dependent, the fact that increased cell proliferation POSTN-mediated was impaired by BIC suggests an androgen modulation of POSTN interaction proteins.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
JournalAnticancer Research
Volume33
Issue number3
Publication statusPublished - Mar 2013

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Dihydrotestosterone
Androgens
Prostatic Neoplasms
Transfection
Cell Line
Growth
Cell Proliferation
Genetic Vectors
Messenger RNA
bicalutamide
Genes
Neoplasms
Proteins

Keywords

  • Androgens
  • Antiandrogens
  • LNCaP cell line
  • Periostin
  • Prostate cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dihydrotestosterone and bicalutamide do not affect periostin expression in androgen-dependent LNCaP prostate cancer cell lines. / Argellati, Francesca; Nuzzo, Pier Vitale; Ricci, Francesco; Mangerini, Rosa; Rubagotti, Alessandra; Boccardo, Francesco.

In: Anticancer Research, Vol. 33, No. 3, 03.2013, p. 815-820.

Research output: Contribution to journalArticle

Argellati, Francesca ; Nuzzo, Pier Vitale ; Ricci, Francesco ; Mangerini, Rosa ; Rubagotti, Alessandra ; Boccardo, Francesco. / Dihydrotestosterone and bicalutamide do not affect periostin expression in androgen-dependent LNCaP prostate cancer cell lines. In: Anticancer Research. 2013 ; Vol. 33, No. 3. pp. 815-820.
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