Abstract
Normal, coumarin, factor II deficient, factor VII deficient, factor X deficient, factor IX deficient, abnormal factor IX and abnormal factor X (factor X Friuli) plasmas were diluted 1:1, 1:2, 1:3, 1:5 and 1:10. Variable aliquots of such dilutions were then added to several tissue thromboplastins and the clotting time measured. Four thromboplastin reagents were used: Thrombotest, Normotest, Manchester Thromboplastin and Simplastin A. Using Manchester Thromboplastin absorbed normal plasma was added to the system too. The "inhibitory effect" present in the system was evaluated by means of conventional units "i". Using Thrombotest, Manchester Thromboplastin or Simplastin A a variable but clear "inhibition" ("i">0.5 units) was found in all plasmas. Using Normotest a slight "inhibition" was present only in coumarin plasmas and in factor VII or factor X deficient plasmas. In factor IX deficient plasma no inhibition could be assessed since in all cases it gave a dilution curve identical to the normal plasma one. The studies indicate that the dilution curve analysis has no value in the case of single congenital prothrombin factors deficiencies since these plasmas "behave" in the system in a way similar to coumarin plasma.
| Original language | English |
|---|---|
| Pages (from-to) | 134-143 |
| Number of pages | 10 |
| Journal | Blut |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 1974 |
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ASJC Scopus subject areas
- Hematology
Cite this
Dilution curve studies in prothrombin complex factors deficiencies and abnormalities. / Girolami, Antonio; Brunetti, Adriano; De Marco, Luigino; Fioretti, Daniele.
In: Blut, Vol. 29, No. 2, 08.1974, p. 134-143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dilution curve studies in prothrombin complex factors deficiencies and abnormalities
AU - Girolami, Antonio
AU - Brunetti, Adriano
AU - De Marco, Luigino
AU - Fioretti, Daniele
PY - 1974/8
Y1 - 1974/8
N2 - Normal, coumarin, factor II deficient, factor VII deficient, factor X deficient, factor IX deficient, abnormal factor IX and abnormal factor X (factor X Friuli) plasmas were diluted 1:1, 1:2, 1:3, 1:5 and 1:10. Variable aliquots of such dilutions were then added to several tissue thromboplastins and the clotting time measured. Four thromboplastin reagents were used: Thrombotest, Normotest, Manchester Thromboplastin and Simplastin A. Using Manchester Thromboplastin absorbed normal plasma was added to the system too. The "inhibitory effect" present in the system was evaluated by means of conventional units "i". Using Thrombotest, Manchester Thromboplastin or Simplastin A a variable but clear "inhibition" ("i">0.5 units) was found in all plasmas. Using Normotest a slight "inhibition" was present only in coumarin plasmas and in factor VII or factor X deficient plasmas. In factor IX deficient plasma no inhibition could be assessed since in all cases it gave a dilution curve identical to the normal plasma one. The studies indicate that the dilution curve analysis has no value in the case of single congenital prothrombin factors deficiencies since these plasmas "behave" in the system in a way similar to coumarin plasma.
AB - Normal, coumarin, factor II deficient, factor VII deficient, factor X deficient, factor IX deficient, abnormal factor IX and abnormal factor X (factor X Friuli) plasmas were diluted 1:1, 1:2, 1:3, 1:5 and 1:10. Variable aliquots of such dilutions were then added to several tissue thromboplastins and the clotting time measured. Four thromboplastin reagents were used: Thrombotest, Normotest, Manchester Thromboplastin and Simplastin A. Using Manchester Thromboplastin absorbed normal plasma was added to the system too. The "inhibitory effect" present in the system was evaluated by means of conventional units "i". Using Thrombotest, Manchester Thromboplastin or Simplastin A a variable but clear "inhibition" ("i">0.5 units) was found in all plasmas. Using Normotest a slight "inhibition" was present only in coumarin plasmas and in factor VII or factor X deficient plasmas. In factor IX deficient plasma no inhibition could be assessed since in all cases it gave a dilution curve identical to the normal plasma one. The studies indicate that the dilution curve analysis has no value in the case of single congenital prothrombin factors deficiencies since these plasmas "behave" in the system in a way similar to coumarin plasma.
UR - http://www.scopus.com/inward/record.url?scp=0016194940&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016194940&partnerID=8YFLogxK
U2 - 10.1007/BF01633837
DO - 10.1007/BF01633837
M3 - Article
C2 - 4851393
AN - SCOPUS:0016194940
VL - 29
SP - 134
EP - 143
JO - Annals of Hematology
JF - Annals of Hematology
SN - 0939-5555
IS - 2
ER -