Dimethyl sulfoxide interferes with in vitro differentiation of chick embryo endochondral chondrocytes

Paola Manduca, Patrizio Castagnola, Ranieri Cancedda

Research output: Contribution to journalArticlepeer-review

Abstract

Dedifferentiated chondrocytes derived from 6-day-old chick embryo tibiae when transferred on agarose, revert to the chondrocytic phenotype and mature to hypertrophic, type X collagen-producing chondrocytes (Castagnola et al. (1986). J. Cell Biol. 102, 2310-2317). The continuous presence of 180 mM dimethyl sulfoxide (DMSO) during the culture specifically inhibited synthesis of type X collagen and accumulation of its mRNA. The synthesis of the cartilage-specific type II collagen and the level of its mRNA were essentially unchanged in treated and control untreated cells.

Original languageEnglish
Pages (from-to)234-236
Number of pages3
JournalDevelopmental Biology
Volume125
Issue number1
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Developmental Biology

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