Dioxin-like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX

Marcella Warner, Brenda Eskenazi, Donald G. Patterson, George Clark, Wayman E. Turner, Luigi Bonsignore, Paolo Mocarelli, Pier Mario Gerthoux

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) are widespread environmental contaminants that exist as complex mixtures and are frequently detected at part-per-trillion (ppt) levels in humans. Using isotope dilution high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS), we measure the PCDDs, PCDFs, and PCBs in serum of a population of 78 women residing in an area near Seveso, Italy where a TCDD explosion occurred in 1976 and where furniture is manufactured. The average total dioxin-like toxic equivalents (TEQ) of these women was 25.3 ppt, lipid-adjusted, comparable to other parts of Europe. TCDD levels, however, were higher among the few women who resided in the exposed area in 1976. We examined the possibility of using the CALUX (chemical-activated luciferase gene expression) bioassay to estimate total TEQ in a small volume of plasma from this population. A total of 32 archived plasma specimens were selected for CALUX bioassay, based on the distribution of Total TEQ by HRGC/HRMS. The CALUX bioassay was performed blind to HRGC/HRMS results with 2 ml plasma per replicate analysis. Of 32 samples, 10 were below detection limits in the CALUX bioassay. For the 32 samples, the CALUX-TEQ averaged 25.4 ppt, lipid-adjusted (range: 0-127.6) and was not significantly different from the HRGC/HRMS Total TEQ average of 31.2 ppt, lipid-adjusted (range: 12.7-88.3) (t = 0.88, P = 0.38). however, the two measures were not significantly correlated (Rs = 0.04, P = 0.82). More validation of the CALUX bioassay with larger sample volume is needed before application as an exposure measure in large-scale epidemiologic studies of health effects of dioxin-like compounds.

Original languageEnglish
Pages (from-to)310-318
Number of pages9
JournalJournal of Exposure Analysis and Environmental Epidemiology
Volume15
Issue number4
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Dioxins
Bioassay
Poisons
Biological Assay
Gas chromatography
Gas Chromatography
dioxin
Italy
Mass spectrometry
Mass Spectrometry
gas chromatography
mass spectrometry
Lipids
bioassay
Polychlorinated Biphenyls
Polychlorinated biphenyls
Plasmas
TCDD
lipid
PCDF

Keywords

  • CALUX
  • PCB
  • PCDD
  • PCDF
  • TCDD
  • TEQ

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Pollution
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Warner, M., Eskenazi, B., Patterson, D. G., Clark, G., Turner, W. E., Bonsignore, L., ... Gerthoux, P. M. (2005). Dioxin-like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX. Journal of Exposure Analysis and Environmental Epidemiology, 15(4), 310-318. https://doi.org/10.1038/sj.jea.7500407

Dioxin-like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX. / Warner, Marcella; Eskenazi, Brenda; Patterson, Donald G.; Clark, George; Turner, Wayman E.; Bonsignore, Luigi; Mocarelli, Paolo; Gerthoux, Pier Mario.

In: Journal of Exposure Analysis and Environmental Epidemiology, Vol. 15, No. 4, 07.2005, p. 310-318.

Research output: Contribution to journalArticle

Warner, M, Eskenazi, B, Patterson, DG, Clark, G, Turner, WE, Bonsignore, L, Mocarelli, P & Gerthoux, PM 2005, 'Dioxin-like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX', Journal of Exposure Analysis and Environmental Epidemiology, vol. 15, no. 4, pp. 310-318. https://doi.org/10.1038/sj.jea.7500407
Warner, Marcella ; Eskenazi, Brenda ; Patterson, Donald G. ; Clark, George ; Turner, Wayman E. ; Bonsignore, Luigi ; Mocarelli, Paolo ; Gerthoux, Pier Mario. / Dioxin-like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX. In: Journal of Exposure Analysis and Environmental Epidemiology. 2005 ; Vol. 15, No. 4. pp. 310-318.
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