Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation

Daniela Salvemini, Waldemar Radziszewski, Vincenzo Mollace, Adrian Moore, Derek Willoughby, John Vane

Research output: Contribution to journalArticle

Abstract

Diphenylene iodonium is an inhibitor of the enzyme NADPH-oxidase and prevents the generation of oxygen-derived free radicals in neutrophils (Cross and Jones, 1986). Here we show that diphenylene iodonium (0.25-2 μm) inhibited, according to the dose, thrombin-induced platelet-aggregation in human washed platelets and ADP-induced platelet aggregation in platelet-rich plasma. At the concentrations which inhibited platelet aggregation diphenylene iodonium did not alter platelet concentrations of cAMP or cGMP but enhanced the anti-platelet activity of iloprost, sodium nitroprusside or cultured endothelial cells. These findings highlight the importance of free radicals as platelet pro-aggregatory agents.

Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalEuropean Journal of Pharmacology
Volume199
Issue number1
DOIs
Publication statusPublished - Jun 18 1991

Keywords

  • Anti-platelet agents
  • Diphenylene iodonium
  • Endothelial cells
  • Free radicals
  • Platelet aggregation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Fingerprint Dive into the research topics of 'Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation'. Together they form a unique fingerprint.

  • Cite this