Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation

Daniela Salvemini, Waldemar Radziszewski, Vincenzo Mollace, Adrian Moore, Derek Willoughby, John Vane

Research output: Contribution to journalArticle

Abstract

Diphenylene iodonium is an inhibitor of the enzyme NADPH-oxidase and prevents the generation of oxygen-derived free radicals in neutrophils (Cross and Jones, 1986). Here we show that diphenylene iodonium (0.25-2 μm) inhibited, according to the dose, thrombin-induced platelet-aggregation in human washed platelets and ADP-induced platelet aggregation in platelet-rich plasma. At the concentrations which inhibited platelet aggregation diphenylene iodonium did not alter platelet concentrations of cAMP or cGMP but enhanced the anti-platelet activity of iloprost, sodium nitroprusside or cultured endothelial cells. These findings highlight the importance of free radicals as platelet pro-aggregatory agents.

Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalEuropean Journal of Pharmacology
Volume199
Issue number1
DOIs
Publication statusPublished - Jun 18 1991

Fingerprint

Platelet Aggregation
Free Radicals
Blood Platelets
Iloprost
Platelet-Rich Plasma
NADPH Oxidase
Nitroprusside
Enzyme Inhibitors
Thrombin
Adenosine Diphosphate
Cultured Cells
Neutrophils
Endothelial Cells
Oxygen
diphenyleneiodonium

Keywords

  • Anti-platelet agents
  • Diphenylene iodonium
  • Endothelial cells
  • Free radicals
  • Platelet aggregation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation. / Salvemini, Daniela; Radziszewski, Waldemar; Mollace, Vincenzo; Moore, Adrian; Willoughby, Derek; Vane, John.

In: European Journal of Pharmacology, Vol. 199, No. 1, 18.06.1991, p. 15-18.

Research output: Contribution to journalArticle

Salvemini, D, Radziszewski, W, Mollace, V, Moore, A, Willoughby, D & Vane, J 1991, 'Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation', European Journal of Pharmacology, vol. 199, no. 1, pp. 15-18. https://doi.org/10.1016/0014-2999(91)90631-Y
Salvemini, Daniela ; Radziszewski, Waldemar ; Mollace, Vincenzo ; Moore, Adrian ; Willoughby, Derek ; Vane, John. / Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation. In: European Journal of Pharmacology. 1991 ; Vol. 199, No. 1. pp. 15-18.
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