Direct assessment of plasma/serum sample quality for proteomics biomarker investigation

Viviana Greco, Cristian Piras, Luisa Pieroni, Andrea Urbani

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Blood proteome analysis for biomarker discovery represents one of the most challenging tasks to be achieved through clinical proteomics due to the sample complexity, such as the extreme heterogeneity of proteins in very dynamic concentrations, and to the observation of proper sampling and storage conditions. Quantitative and qualitative proteomics profiling of plasma and serum could be useful both for the early detection of diseases and for the evaluation of pathological status. Two main sources of variability can affect the precision and accuracy of the quantitative experiments designed for biomarker discovery and validation. These sources are divided into two categories, pre-analytical and analytical, and are often ignored; however, they can contribute to consistent errors and misunderstanding in biomarker research. In this chapter, we review critical pre-analytical and analytical variables that can influence quantitative proteomics. According to guidelines accepted by proteomics community, we propose some recommendations and strategies for a proper proteomics analysis addressed to biomarker studies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-21
Number of pages19
Volume1619
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1619
ISSN (Print)1064-3745

Keywords

  • Biomarkers
  • Clinical proteomics
  • Mass spectrometry
  • Pre-analytical and analytical variables
  • Sample quality control

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Greco, V., Piras, C., Pieroni, L., & Urbani, A. (2017). Direct assessment of plasma/serum sample quality for proteomics biomarker investigation. In Methods in Molecular Biology (Vol. 1619, pp. 3-21). (Methods in Molecular Biology; Vol. 1619). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7057-5_1