Direct detection of Bordetella pertusis and Bordetella parapertussis

Comparison of polymerase chain reaction and culture

Antonella De Donno, M. Quattrocchi, F. Ansaldi, A. Campa, M. C. Rollo, G. Gabutti

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We evaluated the diagnostic performance of a genomic DNA amplification method for Bordetella pertussis and Bordetella parapertussis compared with culture isolation. Aliquots from B. pertussis and B. parapertussis cultures were added to sterile physiological saline or sterile distilled water to give bacterial suspensions of 108 cells/ml and serial dilutions were prepared. Suspensions in physiological saline were cultured on charcoal agar medium; bacterial growth was observed up to dilutions of 10-7. Suspensions in distilled water were subjected to DNA extraction and nested polymerase chain reaction (PCR) was performed on the extracts; the PCR was positive up to dilutions of 10-8 for B. pertussis and 10-9 for B. parapertussis. Since the efficacy of culture isolation, regarded as the standard for the detection of B. pertussis and B. parapertussis, declines after the first stage of pertussis or with prior vaccination or antibiotic therapy, PCR, although not yet standardized, may provide an alternative diagnostic tool.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalJournal of International Medical Research
Volume34
Issue number4
Publication statusPublished - Jul 2006

Fingerprint

Bordetella parapertussis
Bordetella
Bordetella pertussis
Polymerase chain reaction
Dilution
Suspensions
Polymerase Chain Reaction
Water
Charcoal
DNA
Agar
Amplification
Whooping Cough
Anti-Bacterial Agents
Vaccination
Growth

Keywords

  • Bordetella parapertussis
  • Bordetella pertussis
  • Culture
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

De Donno, A., Quattrocchi, M., Ansaldi, F., Campa, A., Rollo, M. C., & Gabutti, G. (2006). Direct detection of Bordetella pertusis and Bordetella parapertussis: Comparison of polymerase chain reaction and culture. Journal of International Medical Research, 34(4), 367-373.

Direct detection of Bordetella pertusis and Bordetella parapertussis : Comparison of polymerase chain reaction and culture. / De Donno, Antonella; Quattrocchi, M.; Ansaldi, F.; Campa, A.; Rollo, M. C.; Gabutti, G.

In: Journal of International Medical Research, Vol. 34, No. 4, 07.2006, p. 367-373.

Research output: Contribution to journalArticle

De Donno, A, Quattrocchi, M, Ansaldi, F, Campa, A, Rollo, MC & Gabutti, G 2006, 'Direct detection of Bordetella pertusis and Bordetella parapertussis: Comparison of polymerase chain reaction and culture', Journal of International Medical Research, vol. 34, no. 4, pp. 367-373.
De Donno, Antonella ; Quattrocchi, M. ; Ansaldi, F. ; Campa, A. ; Rollo, M. C. ; Gabutti, G. / Direct detection of Bordetella pertusis and Bordetella parapertussis : Comparison of polymerase chain reaction and culture. In: Journal of International Medical Research. 2006 ; Vol. 34, No. 4. pp. 367-373.
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