Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method

A. Manzin, S. Paolucci, P. Lampertico, S. Menzo, M. G. Rumi, M. Colombo, M. Clementi

Research output: Contribution to journalArticle

Abstract

A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.

Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalResearch in Virology
Volume144
Issue numberC
DOIs
Publication statusPublished - 1993

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Hepatitis B e Antigens
DNA Sequence Analysis
Hepatitis B virus
Terminator Codon
Population
Virus Diseases
Chronic Hepatitis
Genome
Polymerase Chain Reaction
DNA

Keywords

  • HBV, Pre-core, HBeAg, DNA
  • Sequencing, Mutants

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method. / Manzin, A.; Paolucci, S.; Lampertico, P.; Menzo, S.; Rumi, M. G.; Colombo, M.; Clementi, M.

In: Research in Virology, Vol. 144, No. C, 1993, p. 303-306.

Research output: Contribution to journalArticle

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AU - Rumi, M. G.

AU - Colombo, M.

AU - Clementi, M.

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