Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method

A. Manzin, S. Paolucci, P. Lampertico, S. Menzo, M. G. Rumi, M. Colombo, M. Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.

Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalResearch in Virology
Volume144
Issue numberC
DOIs
Publication statusPublished - 1993

Keywords

  • HBV, Pre-core, HBeAg, DNA
  • Sequencing, Mutants

ASJC Scopus subject areas

  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method'. Together they form a unique fingerprint.

Cite this