Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method

A. Manzin; S. Paolucci; P. Lampertico; S. Menzo; M. G. Rumi; M. Colombo; M. Clementi

doi:10.1016/S0923-2516(06)80045-2

Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method

A. Manzin, S. Paolucci, P. Lampertico, S. Menzo, M. G. Rumi, M. Colombo, M. Clementi

Research output: Contribution to journal › Article › peer-review

Abstract

A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.

Original language	English
Pages (from-to)	303-306
Number of pages	4
Journal	Research in Virology
Volume	144
Issue number	C
DOIs	https://doi.org/10.1016/S0923-2516(06)80045-2
Publication status	Published - 1993

Keywords

HBV, Pre-core, HBeAg, DNA
Sequencing, Mutants

ASJC Scopus subject areas

Immunology
Virology

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title = "Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method",

abstract = "A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.",

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author = "A. Manzin and S. Paolucci and P. Lampertico and S. Menzo and Rumi, {M. G.} and M. Colombo and M. Clementi",

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AU - Manzin, A.

AU - Paolucci, S.

AU - Lampertico, P.

AU - Menzo, S.

AU - Rumi, M. G.

AU - Colombo, M.

AU - Clementi, M.

PY - 1993

Y1 - 1993

N2 - A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.

AB - A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.

KW - HBV, Pre-core, HBeAg, DNA

KW - Sequencing, Mutants

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