Abstract
A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 303-306 |
| Number of pages | 4 |
| Journal | Research in Virology |
| Volume | 144 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- HBV, Pre-core, HBeAg, DNA
- Sequencing, Mutants
ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method. / Manzin, A.; Paolucci, S.; Lampertico, P.; Menzo, S.; Rumi, M. G.; Colombo, M.; Clementi, M.
In: Research in Virology, Vol. 144, No. C, 1993, p. 303-306.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct detection of HBV preC mutants in heterogeneous viral populations by a modified DNA sequencing method
AU - Manzin, A.
AU - Paolucci, S.
AU - Lampertico, P.
AU - Menzo, S.
AU - Rumi, M. G.
AU - Colombo, M.
AU - Clementi, M.
PY - 1993
Y1 - 1993
N2 - A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.
AB - A modified method for the direct sequencing of double-stranded DNA products of PCR amplification is described and has been applied to the analysis of hepatitis B virus (HBV) preC/C region in samples from persistently infected patients with chronic hepatitis. Data was obtained from both hepatitis B e antigen(HBeAg)-positive and -negative chronic carriers. A high prevalence of mixed viral populations (wild-type genomes and mutated sequences with a TAG stop codon in the distal preC region at position 1895-1897) was shown in the HBeAg-positive group; a homogeneous (either mutated or wild-type) viral population was detected in all but one of the long-term HBeAg-negative, untreated chronic carriers, thus suggesting that pre-core mutants can be rapidly generated and selected during the natural course of HBV infection.
KW - HBV, Pre-core, HBeAg, DNA
KW - Sequencing, Mutants
UR - http://www.scopus.com/inward/record.url?scp=0027239454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027239454&partnerID=8YFLogxK
U2 - 10.1016/S0923-2516(06)80045-2
DO - 10.1016/S0923-2516(06)80045-2
M3 - Article
C2 - 8210713
AN - SCOPUS:0027239454
VL - 144
SP - 303
EP - 306
JO - Research in Virology
JF - Research in Virology
SN - 0923-2516
IS - C
ER -