Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients

Alberto Giannetti, Giovanna Zambruno, Andrea Cimarelli, Alessandra Marconi, Matteo Negroni, Giampiero Girolomoni, Umberto Bertazzoni

Research output: Contribution to journalArticle

Abstract

Human Langerhans cells (LC) are bone marrow-derived, HLA-DR+, CDla+, and CD4+ dendritic antigen-presenting cells found in stratified squamous epithelia. As other members of the dendritic leukocyte family, to which they belong, LC have been reported as targets for HIV-1 infection. The aim of the present study was to investigate whether HIV-1 RNA is expressed in epidermal LC of HIV-1-infected patients. Bulk epidermal cell (EC) suspensions were prepared from skin of nine recently deceased AIDS patients and 11 seronegative controls. Purified LC (94 ±4% HLA-DR+ cells with no CD3+cells, as assessed by flow microfluorimetry analysis) and LC-depleted EC were obtained by immunomagnetic separation using an anti-CDla monoclonal antibody. Samples were analyzed for the presence of HIV-1 RNA by reverse transcription of a spliced mRNA region of the tat gene, followed by polymerase chain reaction amplification. HIV-l-spliced RNA was detected in LC from 6 of 9 patients examined, whereas LC-depleted EC fractions from the same patients were all negative. The results indicate that epidermal LC from HIV-seropositive patients actively transcribe HIV-1 proviral DNA, further supporting the hypothesis that HIV productively infected LC could serve as a reservoir of the virus in the epidermis and as a source for the infection of T lymphocytes.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalJournal of Acquired Immune Deficiency Syndromes
Volume6
Issue number4
Publication statusPublished - 1993

Fingerprint

Langerhans Cells
HIV-1
RNA
HIV
HLA-DR Antigens
tat Genes
Immunomagnetic Separation
CD4 Antigens
Antigen-Presenting Cells
Epidermis
Dendritic Cells
Reverse Transcription
HIV Infections
Suspensions
Flow Cytometry
Acquired Immunodeficiency Syndrome
Leukocytes
Epithelium
Bone Marrow
Monoclonal Antibodies

Keywords

  • Epidermal Langerhans cells
  • HIV-1 RNA
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Virology
  • Immunology and Allergy

Cite this

Giannetti, A., Zambruno, G., Cimarelli, A., Marconi, A., Negroni, M., Girolomoni, G., & Bertazzoni, U. (1993). Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients. Journal of Acquired Immune Deficiency Syndromes, 6(4), 329-333.

Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients. / Giannetti, Alberto; Zambruno, Giovanna; Cimarelli, Andrea; Marconi, Alessandra; Negroni, Matteo; Girolomoni, Giampiero; Bertazzoni, Umberto.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 6, No. 4, 1993, p. 329-333.

Research output: Contribution to journalArticle

Giannetti, A, Zambruno, G, Cimarelli, A, Marconi, A, Negroni, M, Girolomoni, G & Bertazzoni, U 1993, 'Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients', Journal of Acquired Immune Deficiency Syndromes, vol. 6, no. 4, pp. 329-333.
Giannetti, Alberto ; Zambruno, Giovanna ; Cimarelli, Andrea ; Marconi, Alessandra ; Negroni, Matteo ; Girolomoni, Giampiero ; Bertazzoni, Umberto. / Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients. In: Journal of Acquired Immune Deficiency Syndromes. 1993 ; Vol. 6, No. 4. pp. 329-333.
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