Abstract
Human Langerhans cells (LC) are bone marrow-derived, HLA-DR+, CDla+, and CD4+ dendritic antigen-presenting cells found in stratified squamous epithelia. As other members of the dendritic leukocyte family, to which they belong, LC have been reported as targets for HIV-1 infection. The aim of the present study was to investigate whether HIV-1 RNA is expressed in epidermal LC of HIV-1-infected patients. Bulk epidermal cell (EC) suspensions were prepared from skin of nine recently deceased AIDS patients and 11 seronegative controls. Purified LC (94 ±4% HLA-DR+ cells with no CD3+cells, as assessed by flow microfluorimetry analysis) and LC-depleted EC were obtained by immunomagnetic separation using an anti-CDla monoclonal antibody. Samples were analyzed for the presence of HIV-1 RNA by reverse transcription of a spliced mRNA region of the tat gene, followed by polymerase chain reaction amplification. HIV-l-spliced RNA was detected in LC from 6 of 9 patients examined, whereas LC-depleted EC fractions from the same patients were all negative. The results indicate that epidermal LC from HIV-seropositive patients actively transcribe HIV-1 proviral DNA, further supporting the hypothesis that HIV productively infected LC could serve as a reservoir of the virus in the epidermis and as a source for the infection of T lymphocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 329-333 |
| Number of pages | 5 |
| Journal | Journal of Acquired Immune Deficiency Syndromes |
| Volume | 6 |
| Issue number | 4 |
| Publication status | Published - 1993 |
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Keywords
- Epidermal Langerhans cells
- HIV-1 RNA
- Polymerase chain reaction
ASJC Scopus subject areas
- Infectious Diseases
- Pharmacology (medical)
- Virology
- Immunology and Allergy
Cite this
Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients. / Giannetti, Alberto; Zambruno, Giovanna; Cimarelli, Andrea; Marconi, Alessandra; Negroni, Matteo; Girolomoni, Giampiero; Bertazzoni, Umberto.
In: Journal of Acquired Immune Deficiency Syndromes, Vol. 6, No. 4, 1993, p. 329-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct detection of HIV-1 rna in epidermal langerhans cells of hiv-infected patients
AU - Giannetti, Alberto
AU - Zambruno, Giovanna
AU - Cimarelli, Andrea
AU - Marconi, Alessandra
AU - Negroni, Matteo
AU - Girolomoni, Giampiero
AU - Bertazzoni, Umberto
PY - 1993
Y1 - 1993
N2 - Human Langerhans cells (LC) are bone marrow-derived, HLA-DR+, CDla+, and CD4+ dendritic antigen-presenting cells found in stratified squamous epithelia. As other members of the dendritic leukocyte family, to which they belong, LC have been reported as targets for HIV-1 infection. The aim of the present study was to investigate whether HIV-1 RNA is expressed in epidermal LC of HIV-1-infected patients. Bulk epidermal cell (EC) suspensions were prepared from skin of nine recently deceased AIDS patients and 11 seronegative controls. Purified LC (94 ±4% HLA-DR+ cells with no CD3+cells, as assessed by flow microfluorimetry analysis) and LC-depleted EC were obtained by immunomagnetic separation using an anti-CDla monoclonal antibody. Samples were analyzed for the presence of HIV-1 RNA by reverse transcription of a spliced mRNA region of the tat gene, followed by polymerase chain reaction amplification. HIV-l-spliced RNA was detected in LC from 6 of 9 patients examined, whereas LC-depleted EC fractions from the same patients were all negative. The results indicate that epidermal LC from HIV-seropositive patients actively transcribe HIV-1 proviral DNA, further supporting the hypothesis that HIV productively infected LC could serve as a reservoir of the virus in the epidermis and as a source for the infection of T lymphocytes.
AB - Human Langerhans cells (LC) are bone marrow-derived, HLA-DR+, CDla+, and CD4+ dendritic antigen-presenting cells found in stratified squamous epithelia. As other members of the dendritic leukocyte family, to which they belong, LC have been reported as targets for HIV-1 infection. The aim of the present study was to investigate whether HIV-1 RNA is expressed in epidermal LC of HIV-1-infected patients. Bulk epidermal cell (EC) suspensions were prepared from skin of nine recently deceased AIDS patients and 11 seronegative controls. Purified LC (94 ±4% HLA-DR+ cells with no CD3+cells, as assessed by flow microfluorimetry analysis) and LC-depleted EC were obtained by immunomagnetic separation using an anti-CDla monoclonal antibody. Samples were analyzed for the presence of HIV-1 RNA by reverse transcription of a spliced mRNA region of the tat gene, followed by polymerase chain reaction amplification. HIV-l-spliced RNA was detected in LC from 6 of 9 patients examined, whereas LC-depleted EC fractions from the same patients were all negative. The results indicate that epidermal LC from HIV-seropositive patients actively transcribe HIV-1 proviral DNA, further supporting the hypothesis that HIV productively infected LC could serve as a reservoir of the virus in the epidermis and as a source for the infection of T lymphocytes.
KW - Epidermal Langerhans cells
KW - HIV-1 RNA
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0027413865&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027413865&partnerID=8YFLogxK
M3 - Article
C2 - 8455138
AN - SCOPUS:0027413865
VL - 6
SP - 329
EP - 333
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
SN - 1525-4135
IS - 4
ER -