Abstract
Polymyxin B is a lipopolysaccharide binding antibiotic used to inactivate potential lipopolysaccharide contaminations when evaluating the activity of different agents on innate immune cells. We report that polymyxin B is able to induce directly in monocyte-derived human dendritic cells (DCs) several functional and molecular modifications characteristic of DCs undergoing a maturation process. DCs incubated with polymyxin B up-regulate the expression of HLA class I and II, the co-stimulatory CD86 molecule, and show an increase in the fraction of adherent cells at short time, which persist at 48 h of incubation. Adhesion to the plate was required for the polymyxin B-induced DCs maturation. A transient activation of IκB-α/NF-κB and ERK1/2 pathways at short time and a further ERK1/2 activation at long term were also detected. Neither up-regulation of the maturation marker CD83 nor activation of p38 nor induction of cytokines secretion was observed in DCs treated with polymyxin B. We demonstrated that inhibition of IκB-α/NF-κB pathway abolishes polymyxin B effects. ERK1/2 inhibition instead allowed DCs treated with polymyxin B to progress in their maturation process as revealed by the increased up-regulation of the CD83 co-stimulatory molecules, the activation of p38, and the reduced adhesion to culture plates at 48 h of incubation. Our results indicate that polymyxin B induces a partial maturation of human DCs through increased adhesion to a substrate and activation of the IκB-α/NF-κB pathway. The increased ERK1/2 activation observed, even though correlating with the initial phases of the maturation process, actually inhibits the occurrence of full maturation.
| Original language | English |
|---|---|
| Pages (from-to) | 14264-14271 |
| Number of pages | 8 |
| Journal | Journal of Biological Chemistry |
| Volume | 280 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - Apr 8 2005 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Direct effects of polymyxin B on human dendritic cells maturation : The role of IκB-α/NF-κB and ERK1/2 pathways and adhesion. / Valentinis, Barbara; Bianchi, Alessandro; Zhou, Dan; Cipponi, Arcadi; Catalanotti, Federica; Russo, Vincenzo; Traversari, Catia.
In: Journal of Biological Chemistry, Vol. 280, No. 14, 08.04.2005, p. 14264-14271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct effects of polymyxin B on human dendritic cells maturation
T2 - The role of IκB-α/NF-κB and ERK1/2 pathways and adhesion
AU - Valentinis, Barbara
AU - Bianchi, Alessandro
AU - Zhou, Dan
AU - Cipponi, Arcadi
AU - Catalanotti, Federica
AU - Russo, Vincenzo
AU - Traversari, Catia
PY - 2005/4/8
Y1 - 2005/4/8
N2 - Polymyxin B is a lipopolysaccharide binding antibiotic used to inactivate potential lipopolysaccharide contaminations when evaluating the activity of different agents on innate immune cells. We report that polymyxin B is able to induce directly in monocyte-derived human dendritic cells (DCs) several functional and molecular modifications characteristic of DCs undergoing a maturation process. DCs incubated with polymyxin B up-regulate the expression of HLA class I and II, the co-stimulatory CD86 molecule, and show an increase in the fraction of adherent cells at short time, which persist at 48 h of incubation. Adhesion to the plate was required for the polymyxin B-induced DCs maturation. A transient activation of IκB-α/NF-κB and ERK1/2 pathways at short time and a further ERK1/2 activation at long term were also detected. Neither up-regulation of the maturation marker CD83 nor activation of p38 nor induction of cytokines secretion was observed in DCs treated with polymyxin B. We demonstrated that inhibition of IκB-α/NF-κB pathway abolishes polymyxin B effects. ERK1/2 inhibition instead allowed DCs treated with polymyxin B to progress in their maturation process as revealed by the increased up-regulation of the CD83 co-stimulatory molecules, the activation of p38, and the reduced adhesion to culture plates at 48 h of incubation. Our results indicate that polymyxin B induces a partial maturation of human DCs through increased adhesion to a substrate and activation of the IκB-α/NF-κB pathway. The increased ERK1/2 activation observed, even though correlating with the initial phases of the maturation process, actually inhibits the occurrence of full maturation.
AB - Polymyxin B is a lipopolysaccharide binding antibiotic used to inactivate potential lipopolysaccharide contaminations when evaluating the activity of different agents on innate immune cells. We report that polymyxin B is able to induce directly in monocyte-derived human dendritic cells (DCs) several functional and molecular modifications characteristic of DCs undergoing a maturation process. DCs incubated with polymyxin B up-regulate the expression of HLA class I and II, the co-stimulatory CD86 molecule, and show an increase in the fraction of adherent cells at short time, which persist at 48 h of incubation. Adhesion to the plate was required for the polymyxin B-induced DCs maturation. A transient activation of IκB-α/NF-κB and ERK1/2 pathways at short time and a further ERK1/2 activation at long term were also detected. Neither up-regulation of the maturation marker CD83 nor activation of p38 nor induction of cytokines secretion was observed in DCs treated with polymyxin B. We demonstrated that inhibition of IκB-α/NF-κB pathway abolishes polymyxin B effects. ERK1/2 inhibition instead allowed DCs treated with polymyxin B to progress in their maturation process as revealed by the increased up-regulation of the CD83 co-stimulatory molecules, the activation of p38, and the reduced adhesion to culture plates at 48 h of incubation. Our results indicate that polymyxin B induces a partial maturation of human DCs through increased adhesion to a substrate and activation of the IκB-α/NF-κB pathway. The increased ERK1/2 activation observed, even though correlating with the initial phases of the maturation process, actually inhibits the occurrence of full maturation.
UR - http://www.scopus.com/inward/record.url?scp=17144426919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17144426919&partnerID=8YFLogxK
U2 - 10.1074/jbc.M410791200
DO - 10.1074/jbc.M410791200
M3 - Article
C2 - 15671028
AN - SCOPUS:17144426919
VL - 280
SP - 14264
EP - 14271
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 14
ER -