Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis

P. Rossi, S. Dolci, C. Albanesi, P. Grimaldi, R. Geremia

Research output: Contribution to journalArticlepeer-review

Abstract

Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at the time of testis determination. We also show that, in the adult male mouse testis, Sry mRNA is expressed at high levels in meiotic and postmeiotic germ cells and, at much lower levels, also in Sertoli cells. Treatment with cyclic adenosine monophosphate (cAMP) analogs of cultured Sertoli cells from postnatal testis completely abolishes Sry mRNA expression.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalMolecular Reproduction and Development
Volume34
Issue number4
Publication statusPublished - 1993

Keywords

  • Embryonal gonad
  • RT-PCR
  • Sertoli cells
  • Sex determination
  • Spermatids
  • Spermatocytes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Genetics

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