Direct identification of microorganisms from positive blood cultures using the lysis-filtration technique and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): A multicentre study

Claudio Farina, Fabio Arena, Patrizia Casprini, Paola Cichero, Massimo Clementi, Marina Cosentino, Roberto Degl'Innocenti, Tommaso Giani, Francesco Luzzaro, Romano Mattei, Carola Mauri, Maria Nardone, Gian Maria Rossolini, Paula Andrea Serna Ortega, Francesca Vailati

Research output: Contribution to journalArticlepeer-review

Abstract

Microbial identification from blood cultures is essential to institute optimal antibiotic therapy and improve survival possibilities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied to identify bacteria and yeasts from positive blood cultures broths. The aim of this multicentre study was to evaluate the reliability of the lysis-filtration technique associated with MALDI-TOF MS to directly identify microorganisms from 765 positive blood cultures collected in six Italian hospitals. Overall, 675/765 (78.1%) blood isolates were correctly identified at the species level, with significant differences between Gram-negative and Gram-positive bacteria (92.6%, and 69.8%, respectively). Some difficulties arise in identifying Streptococcus pneumoniae, Staphylococcus aureus, yeasts and anaerobes. The lysis-filtration protocol is a suitable procedure in terms of performance in identifying microorganisms, but it is quite expensive and technically time-consuming since the time of filtration is not regular for all the samples. The application of the MALDI-TOF MS technique to the direct microbial identification from positive blood cultures is a very promising approach, even if more experience must be gained to minimize errors and costs.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalNew Microbiologica
Volume38
Issue number2
Publication statusPublished - Apr 1 2015

Keywords

  • Blood culture
  • Identification
  • Lysis-filtration technique
  • MALDI-TOF

ASJC Scopus subject areas

  • Microbiology (medical)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Direct identification of microorganisms from positive blood cultures using the lysis-filtration technique and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): A multicentre study'. Together they form a unique fingerprint.

Cite this