Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

David Llères, Marco Denegri, Marco Biggiogera, Paul Ajuh, Angus I. Lamond

Research output: Contribution to journalArticle

Abstract

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5′ and 3′ alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalEMBO Reports
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 2010

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Keywords

  • Alternative pre-mRNA splicing
  • CDC5L
  • FLIM-FRET
  • HnRNP-M
  • Spliceosome

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

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