Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells

Rut Valgardsdottir, Cristina Capitanio, Gemma Texido, Daniela Pende, Claudia Cantoni, Enrico Pesenti, Alessandro Rambaldi, Josée Golay, Martino Introna

Research output: Contribution to journalArticle

Abstract

Cytokine-induced killer (CIK) cells are in-vitro-expanded T lymphocytes that represent a heterogeneous population. A large majority of CIK cells are CD3+CD56+, and this population has been shown to confer a cytotoxic effect against tumor targets. The scope of this work was to study whether CD56 has a direct role in CIK-mediated cytotoxicity. Blocking of CD56 with the anti-CD56 monoclonal antibody GPR165 significantly reduced CIK-mediated lysis of three CD56+ hematopoietic tumor cell lines (AML-NS8, NB4, and KCL22), whereas no effect was observed on three CD56- hematopoietic tumor cell lines (K562, REH, and MOLT-4). Knockdown of CD56 in CIK cells by short interfering RNA made the cells less cytotoxic against a CD56+ target, and knockdown of CD56 in target cells with lentiviral short hairpin RNA significantly altered their susceptibility to CIK-mediated lysis. Our data suggest that homophilic interaction between CD56 molecules may occur in tumor-cell recognition, leading to CIK-mediated cell death.

Original languageEnglish
Pages (from-to)1013-1021
Number of pages9
JournalExperimental Hematology
Volume42
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology

Fingerprint Dive into the research topics of 'Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells'. Together they form a unique fingerprint.

  • Cite this

    Valgardsdottir, R., Capitanio, C., Texido, G., Pende, D., Cantoni, C., Pesenti, E., Rambaldi, A., Golay, J., & Introna, M. (2014). Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells. Experimental Hematology, 42(12), 1013-1021. https://doi.org/10.1016/j.exphem.2014.08.005