Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis

Nicasio Mancini; Cristina M. Ossi; Mario Perotti; Silvia Carletti; Claudia Gianni; Giorgio Paganoni; Stanislav Matuška; Maria Guglielminetti; Annalisa Cavallero; Roberto Burioni; Paolo Rama; Massimo Clementi

doi:10.1099/jmm.0.46029-0

Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis

Nicasio Mancini, Cristina M. Ossi, Mario Perotti, Silvia Carletti, Claudia Gianni, Giorgio Paganoni, Stanislav Matuška, Maria Guglielminetti, Annalisa Cavallero, Roberto Burioni, Paolo Rama, Massimo Clementi

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article

Abstract

The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.

Original language	English
Pages (from-to)	897-900
Number of pages	4
Journal	Journal of Medical Microbiology
Volume	54
Issue number	9
DOIs	https://doi.org/10.1099/jmm.0.46029-0
Publication status	Published - Sep 2005

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ASJC Scopus subject areas

Microbiology (medical)
Microbiology

Cite this

Mancini, N., Ossi, C. M., Perotti, M., Carletti, S., Gianni, C., Paganoni, G., Matuška, S., Guglielminetti, M., Cavallero, A., Burioni, R., Rama, P., & Clementi, M. (2005). Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. Journal of Medical Microbiology, 54(9), 897-900. https://doi.org/10.1099/jmm.0.46029-0

Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. / Mancini, Nicasio; Ossi, Cristina M.; Perotti, Mario; Carletti, Silvia; Gianni, Claudia; Paganoni, Giorgio; Matuška, Stanislav; Guglielminetti, Maria; Cavallero, Annalisa; Burioni, Roberto ; Rama, Paolo ; Clementi, Massimo.

In: Journal of Medical Microbiology, Vol. 54, No. 9, 09.2005, p. 897-900.

Research output: Contribution to journal › Article

Mancini, Nicasio ; Ossi, Cristina M. ; Perotti, Mario ; Carletti, Silvia ; Gianni, Claudia ; Paganoni, Giorgio ; Matuška, Stanislav ; Guglielminetti, Maria ; Cavallero, Annalisa ; Burioni, Roberto ; Rama, Paolo ; Clementi, Massimo. / Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. In: Journal of Medical Microbiology. 2005 ; Vol. 54, No. 9. pp. 897-900.

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10.1099/jmm.0.46029-0