Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis

Nicasio Mancini, Cristina M. Ossi, Mario Perotti, Silvia Carletti, Claudia Gianni, Giorgio Paganoni, Stanislav Matuška, Maria Guglielminetti, Annalisa Cavallero, Roberto Burioni, Paolo Rama, Massimo Clementi

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Abstract

The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.

Original languageEnglish
Pages (from-to)897-900
Number of pages4
JournalJournal of Medical Microbiology
Volume54
Issue number9
DOIs
Publication statusPublished - Sep 2005

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Mancini, N., Ossi, C. M., Perotti, M., Carletti, S., Gianni, C., Paganoni, G., Matuška, S., Guglielminetti, M., Cavallero, A., Burioni, R., Rama, P., & Clementi, M. (2005). Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. Journal of Medical Microbiology, 54(9), 897-900. https://doi.org/10.1099/jmm.0.46029-0