Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer

Claudio Napoli, Antonio Giordano, Amelia Casamassimi, Francesca Pentimalli, Louis J. Ignarro, Filomena De Nigris

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Targeting neoangiogenesis is a well-established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.

Original languageEnglish
Pages (from-to)1505-1508
Number of pages4
JournalInternational Journal of Cancer
Volume128
Issue number7
DOIs
Publication statusPublished - Apr 1 2011

Fingerprint

Silicones
Nude Mice
Neoplasms
Neoplasm Metastasis
Research
Therapeutics

Keywords

  • angiogenesis
  • cancer
  • endothelium

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer. / Napoli, Claudio; Giordano, Antonio; Casamassimi, Amelia; Pentimalli, Francesca; Ignarro, Louis J.; De Nigris, Filomena.

In: International Journal of Cancer, Vol. 128, No. 7, 01.04.2011, p. 1505-1508.

Research output: Contribution to journalArticle

Napoli, Claudio ; Giordano, Antonio ; Casamassimi, Amelia ; Pentimalli, Francesca ; Ignarro, Louis J. ; De Nigris, Filomena. / Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer. In: International Journal of Cancer. 2011 ; Vol. 128, No. 7. pp. 1505-1508.
@article{f029d98f7b954c93817770079abd4b7e,
title = "Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer",
abstract = "Targeting neoangiogenesis is a well-established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.",
keywords = "angiogenesis, cancer, endothelium",
author = "Claudio Napoli and Antonio Giordano and Amelia Casamassimi and Francesca Pentimalli and Ignarro, {Louis J.} and {De Nigris}, Filomena",
year = "2011",
month = "4",
day = "1",
doi = "10.1002/ijc.25743",
language = "English",
volume = "128",
pages = "1505--1508",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "7",

}

TY - JOUR

T1 - Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer

AU - Napoli, Claudio

AU - Giordano, Antonio

AU - Casamassimi, Amelia

AU - Pentimalli, Francesca

AU - Ignarro, Louis J.

AU - De Nigris, Filomena

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Targeting neoangiogenesis is a well-established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.

AB - Targeting neoangiogenesis is a well-established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.

KW - angiogenesis

KW - cancer

KW - endothelium

UR - http://www.scopus.com/inward/record.url?scp=79551496459&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551496459&partnerID=8YFLogxK

U2 - 10.1002/ijc.25743

DO - 10.1002/ijc.25743

M3 - Article

C2 - 21280032

AN - SCOPUS:79551496459

VL - 128

SP - 1505

EP - 1508

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 7

ER -